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[Show more]Predominant form of DNA 
Right-handed B 
 
 
 
DNA and histone interaction is due to what type of bond? 
Ionic 
 
 
 
 
 
 
00:57 
 
01:25 
What stains to the minor groove of DNA duplex? 
SYBR Green 
 
 
 
What is the most abundant type of RNA? 
Ribosomal RNA (rRNA) 
 
 
 
What does RNA act as in a ...
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Add to cartPredominant form of DNA 
Right-handed B 
 
 
 
DNA and histone interaction is due to what type of bond? 
Ionic 
 
 
 
 
 
 
00:57 
 
01:25 
What stains to the minor groove of DNA duplex? 
SYBR Green 
 
 
 
What is the most abundant type of RNA? 
Ribosomal RNA (rRNA) 
 
 
 
What does RNA act as in a ...
Predominant form of DNA 
Right-handed B 
 
 
 
DNA and histone interaction is due to what type of bond? 
Ionic 
 
 
 
What stains to the minor groove of DNA duplex? 
SYBR Green 
 
 
 
What is the most abundant type of RNA? 
Ribosomal RNA (rRNA) 
 
 
 
What does RNA act as in a retrovirus? 
Genome an...
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Add to cartPredominant form of DNA 
Right-handed B 
 
 
 
DNA and histone interaction is due to what type of bond? 
Ionic 
 
 
 
What stains to the minor groove of DNA duplex? 
SYBR Green 
 
 
 
What is the most abundant type of RNA? 
Ribosomal RNA (rRNA) 
 
 
 
What does RNA act as in a retrovirus? 
Genome an...
What is the function of DNA? 
Store genetic information 
 
 
 
Purine 
Double ring of nitrogen base (G, A) 
 
 
 
Pyrimidine 
Single ring of nitrogen base (T, C) 
 
 
 
What is the complementary sequence to 5'AGGTCA3' ? 
5'TGACCT3' 
 
 
 
Which of the ribose carbons participate in the phosphodie...
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Add to cartWhat is the function of DNA? 
Store genetic information 
 
 
 
Purine 
Double ring of nitrogen base (G, A) 
 
 
 
Pyrimidine 
Single ring of nitrogen base (T, C) 
 
 
 
What is the complementary sequence to 5'AGGTCA3' ? 
5'TGACCT3' 
 
 
 
Which of the ribose carbons participate in the phosphodie...
What kind of bond do histones have with DNA? 
Ionic 
 
 
 
What amino acids compose histones? 
Lysine and Arginine 
 
 
 
Which lymphoma has t-cell rearrangements? 
Sezary Syndrome 
 
 
 
Acute Promyelocytic leukemia translocation? 
t(15,17) 
 
 
 
Leading strand polymerase: 
Delta 
 
 
 
DNA repair...
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Add to cartWhat kind of bond do histones have with DNA? 
Ionic 
 
 
 
What amino acids compose histones? 
Lysine and Arginine 
 
 
 
Which lymphoma has t-cell rearrangements? 
Sezary Syndrome 
 
 
 
Acute Promyelocytic leukemia translocation? 
t(15,17) 
 
 
 
Leading strand polymerase: 
Delta 
 
 
 
DNA repair...
What is metagenome? 
Sampling the genome sequence of a community of organisms. 
 
 
 
Polymorphism involved for Irinotecan? 
UGT1A1 
 
 
 
NGS related to deletions and insertions, coverage, depth? 
-Paired-end reads for detection of indels 
-Coverage is how much of your target has been read 
-Depth ...
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Add to cartWhat is metagenome? 
Sampling the genome sequence of a community of organisms. 
 
 
 
Polymorphism involved for Irinotecan? 
UGT1A1 
 
 
 
NGS related to deletions and insertions, coverage, depth? 
-Paired-end reads for detection of indels 
-Coverage is how much of your target has been read 
-Depth ...
Which of the following is not a component of a nucleotide? 
Phosphate group 
Anti-codon 
Ribose sugar 
Nitrogen base 
Anti-codon 
 
 
 
According to Chargaff's rule of base pairing, adenine pairs with: 
Thymine 
 
 
 
What genes would be screened in a breast cancer panel? 
HER2, ERBB2, BRCA1 
 
 
 ...
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Add to cartWhich of the following is not a component of a nucleotide? 
Phosphate group 
Anti-codon 
Ribose sugar 
Nitrogen base 
Anti-codon 
 
 
 
According to Chargaff's rule of base pairing, adenine pairs with: 
Thymine 
 
 
 
What genes would be screened in a breast cancer panel? 
HER2, ERBB2, BRCA1 
 
 
 ...
Acute Promylocity Leukemia (APL) 
t(15:17) 
PML / RARa genes 
 
 
 
Chronic Myelogenous Leukemia (CML) 
t(9:22) 
a.k.a. Philadelphia chromosome 
ABL1 / BCR genes 
 
 
 
 
 
 
01:19 
 
01:25 
Burkitt's non-Hodkin's Lymphoma 
t(8:14) 
dysregulation of c-myc gene 
breakpoints on ch.8 spread over 190k...
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Add to cartAcute Promylocity Leukemia (APL) 
t(15:17) 
PML / RARa genes 
 
 
 
Chronic Myelogenous Leukemia (CML) 
t(9:22) 
a.k.a. Philadelphia chromosome 
ABL1 / BCR genes 
 
 
 
 
 
 
01:19 
 
01:25 
Burkitt's non-Hodkin's Lymphoma 
t(8:14) 
dysregulation of c-myc gene 
breakpoints on ch.8 spread over 190k...
ASCP Molecular Biology Exam Prep|2023 LATEST UPDATE|GUARANTEED SUCCESS
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What does barcoding do in Next Generation Sequencing? 
Adds unique tag to all nucleic acids within one sample so that various samples can be mixed 
 
 
 
What is the best way to store RNA for longer than a month? 
In Ethanol, at -20°C or -80°C 
 
 
 
Testing for Aldehyde Dehydrogenase deficiency i...
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Add to cartWhat does barcoding do in Next Generation Sequencing? 
Adds unique tag to all nucleic acids within one sample so that various samples can be mixed 
 
 
 
What is the best way to store RNA for longer than a month? 
In Ethanol, at -20°C or -80°C 
 
 
 
Testing for Aldehyde Dehydrogenase deficiency i...
The enzyme that primes DNA synthesis is: 
DNA primase 
 
 
 
Which of the following is a common hydrogen bond acceptor? 
 
a) A carbonyl 
b) Primary amine 
c) Hydroxyl 
d) Tertiary amine 
b) Primary amine 
 
 
 
What assay is a signal amplification test and uses Alkaline Phosphatase? 
 
a) Branched ...
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Add to cartThe enzyme that primes DNA synthesis is: 
DNA primase 
 
 
 
Which of the following is a common hydrogen bond acceptor? 
 
a) A carbonyl 
b) Primary amine 
c) Hydroxyl 
d) Tertiary amine 
b) Primary amine 
 
 
 
What assay is a signal amplification test and uses Alkaline Phosphatase? 
 
a) Branched ...
What is translocation is associated with Burkitt's Lymphoma? 
 
a. t(18; 14) 
b. t(9; 22) 
c. t(8; 14) 
d. t(15; 17) 
t(8; 14) 
 
TIP: Burkkitt's 8 letters, 
 
 
 
Locus PF Child Paternity Index 
 
LOC-A1 3 2/3 2.18 
LOC-B2 7/5 5 0.798 
LOC-C3 17/17 9/17 5.21 
LOC-D4 12 12 1.37 
 
Based on the dat...
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Add to cartWhat is translocation is associated with Burkitt's Lymphoma? 
 
a. t(18; 14) 
b. t(9; 22) 
c. t(8; 14) 
d. t(15; 17) 
t(8; 14) 
 
TIP: Burkkitt's 8 letters, 
 
 
 
Locus PF Child Paternity Index 
 
LOC-A1 3 2/3 2.18 
LOC-B2 7/5 5 0.798 
LOC-C3 17/17 9/17 5.21 
LOC-D4 12 12 1.37 
 
Based on the dat...
What are the 3 types of gel electrophoresis 
Agarose 
Polyacrylamide 
Pulse Field 
 
 
 
What is gel electrophoresis 
the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. 
 
 
 
which direction does the DNA flow in gel electropho...
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Add to cartWhat are the 3 types of gel electrophoresis 
Agarose 
Polyacrylamide 
Pulse Field 
 
 
 
What is gel electrophoresis 
the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. 
 
 
 
which direction does the DNA flow in gel electropho...
What is significant about this protein: LGEKKWCLRVNPKGLDESKDYLSLKSKTLLL 
there is a leucine residue at sequential 7th positions forming a leucine zipper, found in transcription factors. A leucine zipper is a specialized secondary structure of protein.
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Add to cartWhat is significant about this protein: LGEKKWCLRVNPKGLDESKDYLSLKSKTLLL 
there is a leucine residue at sequential 7th positions forming a leucine zipper, found in transcription factors. A leucine zipper is a specialized secondary structure of protein.
1. Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? 
A. 5'-ATCTATGTCGGCAATT-3' 
B. 5'-TTAACGGCTGTATCTA-3' 
C. 5'-AATTGCCGACATAGAT-3' 
D. 5'-GAGCACGCTATCTTAT-3' 
A. 5'-ATCTATGTCGGCAATT-3' 
 
 
 
2. Consider the probe sequence, CTACCGTAATATT...
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Add to cart1. Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? 
A. 5'-ATCTATGTCGGCAATT-3' 
B. 5'-TTAACGGCTGTATCTA-3' 
C. 5'-AATTGCCGACATAGAT-3' 
D. 5'-GAGCACGCTATCTTAT-3' 
A. 5'-ATCTATGTCGGCAATT-3' 
 
 
 
2. Consider the probe sequence, CTACCGTAATATT...
The bond between a phosphate group and the ribose sugar group in RNA is called which of the following? 
(A) Phosphodiester linkage 
(B) Glycosidic bond 
(C) Peptide bond 
(D) Amide bond 
B. Glycosidic bond 
 
 
 
Which of the following is does not play a role in DNA replication? 
(A) DNA polymerase ...
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Add to cartThe bond between a phosphate group and the ribose sugar group in RNA is called which of the following? 
(A) Phosphodiester linkage 
(B) Glycosidic bond 
(C) Peptide bond 
(D) Amide bond 
B. Glycosidic bond 
 
 
 
Which of the following is does not play a role in DNA replication? 
(A) DNA polymerase ...
Which of the following terms refers to the proportion of individuals affected by an autosomal dominant condition that displays manifestations? 
A. codominance 
B. penetrance 
C. expressivity 
D. influence 
E. passivity 
Answer Key: B penetrance 
 
Feedback: Certain autosomal dominant conditions are ...
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Add to cartWhich of the following terms refers to the proportion of individuals affected by an autosomal dominant condition that displays manifestations? 
A. codominance 
B. penetrance 
C. expressivity 
D. influence 
E. passivity 
Answer Key: B penetrance 
 
Feedback: Certain autosomal dominant conditions are ...
Which PCR method has the highest specificity? 
 
A. qPCR 
B. Touchdown PCR 
C. Nested PCR 
D. Two-step PCR 
Nested PCR 
 
 
 
Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? 
 
A. 5'-ATCTATGTCGGCAATT-3' 
B. 5'-TTAACGGCTGTATCTA-3' 
C. 5'-AATTGC...
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Add to cartWhich PCR method has the highest specificity? 
 
A. qPCR 
B. Touchdown PCR 
C. Nested PCR 
D. Two-step PCR 
Nested PCR 
 
 
 
Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? 
 
A. 5'-ATCTATGTCGGCAATT-3' 
B. 5'-TTAACGGCTGTATCTA-3' 
C. 5'-AATTGC...
Molecular Techniques are designed for: 
Nucleic Acids, DNA, & RNA 
 
 
 
DNA Macromolecules are made of: 
Carbon 
Nitrogen 
Oxygen CNOPH 
Phosphorous 
Hydrogen 
 
 
 
The DNA macromolecules form units called: 
nucleotides 
 
 
 
Nucleotides are composed of: 
A phosphorylated ribose sugar & nitrogen ...
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Add to cartMolecular Techniques are designed for: 
Nucleic Acids, DNA, & RNA 
 
 
 
DNA Macromolecules are made of: 
Carbon 
Nitrogen 
Oxygen CNOPH 
Phosphorous 
Hydrogen 
 
 
 
The DNA macromolecules form units called: 
nucleotides 
 
 
 
Nucleotides are composed of: 
A phosphorylated ribose sugar & nitrogen ...
What is CML 
Chromic Myelogenous Leukemia 
Chromosome: N/A 
Translocation: 11:22 or 9:22 
Methods of diagnosis: FISH:CISH 
 
 
 
What is EWS 
EWINGS Sarcoma/ cancer, solid tumors 
Chromosome: N/A 
Translocation: 11:22 
Methods of Diagnosis: RTPCR 
 
 
 
What is ALL 
Acute Lymphoblastic Leukemia 
Chr...
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Add to cartWhat is CML 
Chromic Myelogenous Leukemia 
Chromosome: N/A 
Translocation: 11:22 or 9:22 
Methods of diagnosis: FISH:CISH 
 
 
 
What is EWS 
EWINGS Sarcoma/ cancer, solid tumors 
Chromosome: N/A 
Translocation: 11:22 
Methods of Diagnosis: RTPCR 
 
 
 
What is ALL 
Acute Lymphoblastic Leukemia 
Chr...
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