TSI (Triple Sugar Iron Agar)/ Micro Lab Tests Compilation Bundle

List the tests that are involved with TSI. correct answer: H2S Gas and Carbohydrate Fermentation What is the media for H2S Gas? correct answer: TSI agar What method do you use to place content on the media? correct answer: stab and drag motion what is the substrate for H2S gas? correct a...

What does TSI mean? correct answer: Triple Sugar Iron What is a TSI (Triple Sugar Iron) Test also referred to as? correct answer: Kligers Iron Test What does a TSI Test, test for? correct answer: Bacteria's ability to: -Ferment glucose and lactose/sucrose -Produce gas -Reduce Sulfur ...

For what bacteria would you use the TSI test? correct answer: Gram negative bacilli such as enterobacteriaceae. How would you identify an acidic slant? correct answer: If the slant goes from red to yellow on the phenol red. Why is there more lactose and sucrose in TSI agar than glucose? corr...

TSI stands for________________________? correct answer: Triple Sugar Iron What is the sulfur source in TSI agar? correct answer: Sodium thiosulfate A red slant and a red butt with TSI agar indicates that the microorganism ________? correct answer: Cannot ferment A red slant and a yellow ...

What sugars does triple sugar iron agar contain? correct answer: 1% Lactose 1% Sucrose 0.1% Glucose (Dextrose) What is TSI used for? correct answer: To differentiate enterics based on the ability to reduce sulfur and ferment carbohydrates What is fermented with a red (alkaline) slant and a...

TSI stands for correct answer: Triple Sugar Iron If peptone is metabolized then what is made correct answer: Ammonia What are the 3 carbohydrates present for TSI correct answer: Glucose Sucrose Lactose Glucose is a correct answer: monosaccharide Sucrose and Lactose are correct answer...

Phenylethyl alcohol agar PAA correct answer: Selective media- used to isolate specific groups of bacteria Isolation of mostly Gram positive organisms ** Phenylethyl alcohol ** inhibits Gram negative organisms colonies may be visible , but smaller in number and size Phenylethyl alcohol a...

FeCl3 Used in a PAA agar to detect presence of phenylpyruvic acid positive green color. MAC agar What stain do you do on Day 1 of the unknown lab? You may Gram stain from your unknown broth to confirm if the bacteria are Gram-positive or Gram-negative. A throat culture performed by using ...

Organisms that require specialized environments due to complex nutritional requirements are known as...? correct answer: fastidious organisms The blood agar plate (BAP) is an enrichment medium that tests for fastidious organisms such as? correct answer: Streptococcus spp. (species) The blood...

TSI Agar correct answer: *stab and drag *yellow= acid production/ fermentaion of the sugars in the slant/butt. *red= negative *black= H2S production (salmonella or proteus) what are we looking for in the Indole test after adding kovacs reagant? correct answer: * a red ring which indicates th...

*Selective:* Selects for: Gram (+) *Components:* Phenylethyl alcohol (used for isolation of Gram + from mixed cultures) Plate A is a PEA plate with a Gram positive organism on the left and a Gram negative organism on the right. Plate B is a nutrient agar plate illustrating normal growth of ea...

Why is the catalytic activity of enzymes essential to ensure and regulate cellular metabolism? correct answer: enzymes lower the activation energy for chemical rxn. Why are microorganisms able to cause diary products, such as milk, to sour or curdle? correct answer: microorganisms can break dow...

What does TSI stand for? correct answer: Triple Sugar Iron How do you inoculate a TSI? correct answer: Stab and Drag What does a TSI slant agar select for? correct answer: Differentiates between non-fermenters, glucose fermenters and fermenters of lactose and/or sucrose. It is used to ident...

TSI correct answer: Triple Sugar Iron Yellow= acid production or fermentation of the sugars in the slant or butt. Gas production= indicated by the agar lifting up or breaking Red= negative or uninoculated Black= H2S production due to FeS precipitation (think salmonella or proteus) The ...