Class notes
Lecture Notes
- Course
- Institution
Lecture notes of 13 pages for the course Cell Biology and developmental genetics at QMUL
[Show more]
1 review
By: betulalm • 3 year ago
Seller
Follow
fatima-attia
Reviews received
Content preview
Growth factor receptor endocytic traffickingLearning objectives:
At the level of receptor tyrosine kinase. All receptor tyrosine kinase are GF receptors. Will be looking at the trafficking
of the RTK in the cell
1. What is RTK trafficking
2. Endocytosis
3. Intracellular trafficking
4. Degradation of receptor
5. Ubiquitination endocytosis, degradation
6. Emerging concepts (link trafficking to neurological function)
a. Compartmental signalling
b. RTK nuclear localisation
1- Receptor tyrosine kinase trafficking
Receptor trafficking
There are 2 things that can happen when a ligand binds to a receptor:
1. When ligand binds to receptor = ligand activation, which leads to signalling cascade (left)
2. Internalisation is also called endocytosis (need to know both words) (right)
Receptors are hydrophobic molecules as they are found embedded in the lipid bilayer and therefore when receptors
are internalised they are internalised in vesicles.
Inside the cell the receptor traffics in vesicles that lead to degradation
Membrane compartments
Order of trafficking:
1. Plasma membrane
2. Early endosomes
3. Late endosomes
4. lysosomes
5. Golgi
6. ER
Endosomes are specialised vesicles
The first compartment the receptor enters is the early endosome
The early endosome is also called the secretory endosome that will continue to traffic until reach late endosome
The differences between the early and late endosome:
- Different markers on surface called RAB molecules
- Early endosome contains RAB 5 while the late endosome is enriched with RAB 7
, The receptor in the late endosome then goes into the lysosome to be degraded as the lysosome is acidic and contains
many enzymes.
There is a secretion pathway, which is the opposite to the RTK trafficking pathway
The communication between the organelles is mediated by vesicles
Outside the cell
Membrane compartments communication: membrane vesicles
Cells are not static but rather dynamic
Cargo and receptor consistently traffic in the cell
Membrane compartments communicate between vesicles
7 Steps
1. Formation
- Formation/invagination of the membrane to form a bud
2. Budding
- Is the formation of a bud
3. Pinching off
- To form a vesicle
4. Transport
- Transported inside the cell along microtubules
5. Docking
- Doc to another endosome
6. Fusion
- With membrane of other (late) endosome
7. Unloading
- Deliver whatever is inside the vesicles. In this case, RTK
Endosome
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller fatima-attia. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $3.88. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
66475 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now