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Introduction to proteomics - notes from fundamentals course
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Notes on the introduction of proteomics
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PROPROTEOMICS
, PROTEOMICS - INTRODUCTION
Proteomics is the large scale of study of proteins, particularly their function and
structure. Proteomics is an excellent approach for studying changes in
metabolism in response to different stress conditions. In the present review
focused on different types of techniques for the analysis of expressed proteins.
The techniques include 2-D gel electrophoresis, MALDI-TOF/MS etc., play a
vital role for the analysis of novel proteins and their role in disease maintenance
and treatment. The review also concentrated on applicative perspective of
proteomics in the fields of biomedical, agriculture and food.
MASS SPECTROMETRY – INSTRUMENTS
The molecules from a sample are ionized and converted to the gas phase,
followed by separation according to their mass/charge (m/z) ratio -> NOT
ONLY MASS! Hence, after ionisation of a molecule by the addition of H+ ions,
the m/z ratio of the molecule is measured. The same hold for the opposite when
H+ is removed.
The essential steps in mass spectrometry are:
1) Production of ions in the gas phase
2) Acceleration of ions in an electric field
3) Separation of ions by a mass analyser
4) Detection of each ion with a specific m/z ratio
5) The whole instrument has a high vacuum: 10-6Torr (non-SI unit of
pressure)
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