Class notes
Advanced Immunology class notes
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All of the Advanced immunology notes for 2nd year biomedical science. numbered in order of unit which allows an easy flow
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Immunology 13Tissue culture and In-vivo modelling
In-vivo = inside an organism
In-vitro = outside a whole organism (tissue samples)
In-vivo models:
Immune system = ‘Whole Body’ system
Humans:
- Complex
- Polymorphism in MHC & NK cell receptors
- Gene recombination of BCR & TCR
- People will be exposed to different pathogens throughout life
- Different environmental conditions
Twins will not have an identical immune response
MICE:
- Good understanding of genes
- Quick reproduction period
- Similar immune system to humans
- Can be inbred to reduce genetic differences
- Small in size
- Easy to handle
- Fairly simple genetics
- Least sentient (self aware) animal to practice research on
ETHICS:
3R’s
- Refinement
- Reduction
- Replacement
2 Main strains of mice used:
C57BL/6 mice
- Black
BALB/C Mice
- White
MHC:
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