Summary
Samenvatting Studeertabel Pseudomonas aeruginosa
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Tabel met ALLE voedingsbodems + afbeeldingen voor onderzoek naar Pseudomonas
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Onderzoek naar Pseudomonas aeruginosa- Algemene kenmerken o ! soorten
o G – staafje ▪ P. aeruginosa
o Psychofriel karakter • Infecties in ziekenhuizen
o Beweegelijk ~ polair • Resistent <-> AB & antiseptica
o Oxidase + • Slijmvormer op vless
o Geen suikervergisters → La, Su & Glu – ▪ P. fluorescens
o ! soorten → pigmenten ▪ P. stutzeri
o Watersaprofyten • Rough kolonies
o Storend werken → groei op • Gerimpeld opp.
▪ Mac conkey-agar -> kleurloos
▪ S-S-agar
▪ EMA
▪ Tergitol-7-agar
▪ Endo-agar
Isolatie
Voedings-bodem Inhibitor(en)/remstoff Substraat Indicator Incubatie- Beoordeling Verklaring van het uitzicht na
en (substraten) (indicatoren) omstandig- incubatie (welke omzettingen zijn er
heden al dan niet gebeurd: enzymen, pH-
wijziging, ...)
Pseudomonas CN-supplement OF Gelatine 30° 24-48h Wit-kleurloos / blauwgroen-bruin / IEDERE GROEI = Pseudomonas
Selective agar CFC-spl. caseïne fluoriscerend aeruginosa
-> CN-supplement = cetrimide & nalidixinezuur → isolatie Pseudomonas aeruginosa
-> CFC-supplement = cetrimide, fucidine & cefaloridine → isolatie Pseudomonas
Cetrimide = cetyltrimethylammoniumbromide ~ quaternair NH4+-zout
Isolatie
Voedings-bodem Substraat Indicator Incubatie- Beoordeling Verklaring van het uitzicht na
(substraten) (indicatoren) omstandig-heden incubatie
Oxidase test
King A Glycerol Koelkast (5°C) + 42°C P. aeruginosa 5°C -> groei P. fluorescens
- groenblauw → vorming pyocyanine 42°C -> groei P. aeruginosa
- rood → vorming pyorubine
+ 2 ml chloroform -> schudden -> blauw
King B Glycerol Koelkast (5°C) + 42°C Geelgroen → vorming pyoverdine-fluoresceïne
- onoplosbaar in chloroform
- prd’ie dr P. aeruginosa & fluorescens
TSI Vergisten geen suikers → geel
Zwart = dr pyocyanine
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