Summary
Samenvatting Studeertabel onderzoek naar Salmonella
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Alle voedingsbodems voor onderzoek naar de bacterie Salmonella + eigenschappen en kenmerken van de bacterie
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Isolatiemedium SalmonellaKenmerken
- G- staafjes - Gasvorming KIA
- Katalase + - LA-
- Oxidase – - Su-
- Urease – - Glu+
- Beweegelijk - Lysinedecarboxylase + (-)
- Indool – - Fenylalaninedeaminase –
- H2S+ - Groene kolonies → CLED
- + agglumentatie m. salmonella anti-serum
Voedings-bodem Inhibitor(en)/rem Geremde Substraat Indicator Incuba Beoordeling Verklaring v
stoffen bacteriën tie
Rappaport-Vassiliadis- MgCl2 G- 42°C Dr incubatie bij 43°C w
bouillon malachietgroen G- & Shigella selectiviteit bevordert.
Bismuthsulfiet-agar Bismuthsulfiet; La + anaeroben Glu Bi2(SO3)3 Salmonelle vercaht = zwart,bruine & Fe2+ + Bi3+ → FeS↓ + Bi2S3↓
Bi2(SO3)3 G- FeSO4 groen glimmende kolonies
Briljantgroen
SS-agar; Salmonella- Galzouten G+ Lactose (C- NR Salmonella-> doorschijnende kolonies Salmonella (E) & Shigella = La
Shingella-agar Briljantgroen La+ anaeroben bron) m. zwart centrum –
Shigella-> doorschijnde kolonies
, Proteus & citrobacter -> lichtroze
kolonies m. grijszwart centrum
La+ = roos-rode kolonies
Mac Conkey agar Galzouten G+ Lactose (C- NR Salmonella spp. -> licht geelachtige tot Ook coliformen onderzoek
Nr. 3 = G+ bron) kleurloze kolonies Gn H2S-detectie
kristalviolet E. coli -> rode kolonies
Pseudomonas aeruginosa ->
groenbruine kolonies - fluorescerend
Coliformen → rode kolonie
Nt-coliformen → licht geel-
kleurloos
MLCB; Mannitol- Kristalviolet G+ Mannitol (C-bron) H2S- H2S + -> paarszwarte kolonies
Lysine-kristalviolet- Briljantgroen La + anaeroben Lysine.HCl detectie H2S - -> paarsgrijze kolonies . ingezakt
briljantgroen agar centrum
Salmonella
- MN+
- H2S+ (- = S. Tuphius)
- Lysinedecarboxylatie +
XLD; Xylose-Lysine- Na-deoxycholaat G+ & La + Lysine (5 g/l) FR Salmon. -> Rode kolonies m. zwart Niet autoclaveren
Deoxycholaat Enterobacteriea Xylose (3,75 g/l) H2S- centrum 2x zoveel lactose & surose ivg
ceae Lactose (7,5 g/l) detectie - Vormt zuur uit xylose xylose
Sucrose (7,5 g/l) (Na2S2O3 en - Decarboxyleert Lysine
Fe(III)zout) - Lysine>xylose -> basisch -> rood
Shig.-> rode kolonies
- Zuurv. Uit suikers
- H2S- & Lysine-
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