Summary
Samenvatting Studeertabel Listeria Onderzoek
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ALLE voedingsbodems met eigenschappen en kenmerken voor het onerzoek naar Listeria
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Listeria monocytogenesKenmerken
G + bacterie
Niet sporenvormend staafje
Facultatief anaeroob
Bewegelijk
Isolatie
Voedings-bodem remstoffen Geremde bacteriën Substraat Indicator Incubatie- Beoordeling Verklaring van het uitzicht na
Inhibitor(en) (substraten (indicatoren) omstandi incubatie (welke omzettingen zijn er al
) gheden dan niet gebeurd: enzymen, pH-
wijziging, ...)
Palcam- LiCl G- Mannitol Aesculine 24h bij Listeria zwarte kolonies m. kratertje
medium ~ Polymyxine B G- Fe3+- 37°C * MN – fenolrood blijft rood
Fraser bouillon Acriflavine- ammoniu * Aesculine + zwarte kolonies
hydrochloride mcitraat
ceftazidime Fenolrood
ALOA-medium LiCl G– X- 24 h bij Listeria spp. X-glucoside = detectie v. β-
~ Agar Listeria Nalidixinezuur AB <-> G – glucoside 37°C lichtgroen-groene glucosidase-EZ
acc. To Ceftazidimi AB <-> G + α- kolonies * aanwezig in alle
Ottavioni & Cycloheximide kiem’n fosoftidyl Listeria m. Listeria spp.
agosti ~ fraser Polymixine B G -- inositol lichtgroen + halo * ook in Enterococcus
bouillon spp.
α-fosoftidylinositol lipase
activiteit
* enkel in Listeria m.
Bevestiging
VB Substraa Indicator incubati Beoordeling
t e
Trypticase soy agar m. 5% schapenbloed 24h bij Listeria m. = smalle zone v. heldere haemolyse ~ β-
37° haemolyse
* beter waarnemen haemolyse -> CAMP test
uitvoeren m.
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