Onderzoeksvraag: Wat is het pH-optimum (op 0.1 nauwkeurig) van enzym 1, gemeten bij 37℃ met een incubatietijd van 30 minuten?
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Dit practicum is afgenomen op de computers op school
Onderzoeksvraag:
Wat is het pH-optimum (op 0.1 nauwkeurig) van enzym 1, gemeten bij 37℃ met een incubatietijd
van 30 minuten?
Meetgegevens
Temperatuur = 37℃
Substraatvolume = 6 mL
Enzymvolume = 3 mL
Incubatietijd = 30 minuten
Controle of de top tussen de 60% en 75% ligt door de product concentratie van de top door het
totaal product concentratie te delen en te vermenigvuldigen met 100% om het product
percentage te krijgen:
7932 : 12000 x 100% = 66,1%
Top bij 3 pH-eenheden
Minimum: 8.4 - 1.5 = 6.9
Maximum: 8.4 + 1.5 = 9.9
Afronden op gehele getallen geeft pH(7.0-10.0)
Printscreens
Afbeelding 1: printscreen van enzym 1 pH(0-14)
Afbeelding 2: printscreen van enzym 1 pH(0-14)
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