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Zusammenfassung Ablauf einer PCR
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Ablauf einer PCR (gezielte Vervielfältigung von DNA-Abschnitten)1. Auftrennen des DNA-Doppelstranges durch Schmelzen (92°C-95°C) in
Einzelstränge durch Lösung der Wasserstoffbrückenbindungen zwischen den
komplementären Basen der DNA Denaturierung
2. Zugabe und Anlagerung von komplementären Primern (DNA-Nucleotide)
jeweils am 3`Ende beider DNA-Einzelstränge (freie OH- Gruppe am C3-Atom
des Zuckers Desoxyribose) bei 50°C-60°C) Hybridisierung
3. Zugabe einer hitzestabilen „Taq-Polymerase“ aus dem Bakterium thermus
aquaticus .
Synthese der beiden Tochterstränge entlang der beiden Einzelstränge (von
3`5`) bei 72°C Synthese
Diese Reaktionsfolge wird bis zu 35-mal wiederholt, um genügend Genkopien zu
erhalten.
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