Corresponding to IGCSE Edexcel Pearson Biology specification (information likely to apply to/cover other exam boards).
Highly detailed notes, includes step-by-step methods and examples (where helpful to topic) and annotated diagrams.
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More efficient than
cuttings
Many plantlets produced
from one parent plant.
Can take place any time of
year.
Offspring are genetically
identical to parent plant.
Method:
1. Plant tissue (e.g. cauliflower) is placed into agar. Shoots being to grow.
2. Once shoot growth occurs, the (cauliflower) tissue is placed in a culture medium
containing minerals ions, nutrients and plant hormones needed for root growth. The
environment should be sterile.
3. Once shoot and root growth have occurred, plantlets are placed in a greenhouse.
Greenhouse conditions should be optimum for plants.
E.g. increased humidity, light intensity, optimal temperature for enzymes.
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