Objective: To profile bacterial populations based on hey enzymatic reactions
and determine growth/metabolic characteristics.
*Oxidase Test: Qualitative test to determine the presence or absence of
cytochrome c oxidase activity in bacteria.
Procedure
1. Using a sterile loop pick an isolated colony from BAP.
2. Smear directly onto the reactions of areas of the E
3. Examine test area for color change within 20 seconds.
m
er as
co
eH w
*Catalase Test: Qualitative test to determine the presence or absence of
o.
catalase, an enzyme used to break down hydrogen peroxide.
rs e
ou urc
Procedure
1. Using a sterile loop carefully pick colony from plate.
o
2. Smear colony directly onto the glass slide.
aC s
vi y re
3. Add 2 drops of hydrogen peroxide to each smear.
*Coagulase Test: Qualitative test to determine the presence or absence of
ed d
coagulase, an enzyme that plays a role in the formation of blood clots.
ar stu
Procedure
1. Using a sterile loop carefully pick colony from plate.
is
2. Add colony to tube of rabbit plasma, incubate overnight at 37
Th
degrees Celsius.
3. Next day, example sample for precipitants.
sh
*Lipase Test: Qualitative test to determine the presence or absence of
lipase, an enzyme that hydrolyzes triglycerides (fatty acids/lipids)
Procedure
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