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Basic Cell Factory Design summary, wur, BPE22306
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A summary of the reader of Basic Cell Factory Design wat WUR. This course is given in year 2 of the bachelor Biotechnology.
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Basic Cell Factory Designsummary
By Emma Burgwal
Units: if an atom contains 1 C-atom, 1 mole of material is also 1 C-mole of material. Glucose (1 mole
of material ) has 6 C-moles, because it has 6 C-atoms.
Stoichiometric models
These type of models allow you to translate the production rate of one component into those of
others. They are based on atom, electron, NADH and ATP balances.
Cells require a carbon source, a nitrogen source, a phosphorous source and a sulphur source to
synthesize cell components and products, plus metal ions for the active centre of their enzymes or
, for maintaining gradients. Animal cells need amino acids. An energy source can be divided into two
groups: light or redox reactions (electron donor and acceptor required).
r is the production rate of component i. By using +-signs instead of arrows, you don’t have to decide
whether a component is a reactant or a product. Some compounds can namely be both.
Cells need energy for synthesis of cell components, product synthesis and maintenance. Pirt
rX r
published a model for this: r E=−( + P +mE C X ), where Y is the measured yield factor of cell
Y XE Y PE
mass or product, where m is the maintenance coefficient and where C is the cell mass concentration.
rP
The factor can be removed when there is no product formation which requires an extra energy
Y PE
source (for cell components or waste products from
ATP generation).
Pirt’s law can be written as a function of specific rates.
The simple equations give a linear growth graph. If a
cell does require an energy source for product
formation, the graph gets a different shape. The
specific rate in cells is called μ.
You can use very simple models (see above) for the product formation rate of cell components and
waste products of the ATP generation.
The formula for product formation rate was deduced from experiments by Luedeking and Piret. Qp
(from the specific rate function) can be coupled to μ, which gives the following formula:
This can be substituted into Pirt’s law.
1 Y PX m
Pirt’s and Luedeking-Piret laws can be combined, giving: q S = ( +
Y XS Y PS)μ+( mS + P ).
Y PS
Some facts about production formation:
It gives a decrease in μ at
low specific sugar uptake
rate,
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