100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Verslag Spectrofotometrie - Fe2+ $9.80   Add to cart

Essay

Verslag Spectrofotometrie - Fe2+

 113 views  2 purchases
  • Course
  • Institution

Essay van 5 pagina's voor het vak Labportofolio aan de Artesis (resultaat: 14,5/20)

Preview 2 out of 5  pages

  • December 1, 2021
  • 5
  • 2020/2021
  • Essay
  • Unknown
  • B
avatar-seller
Verslaggevers: Groep:
Lector:
Labportfolio: algemene chemie 2
Beoordeling: 14,5/20

Colorimetrie: spectrofotometrie Fe2+
1. Doel

lmax bepalen aan de hand van een extinctiecurve. Stel bij deze golflengte de ijklijn op aan de hand van
een colorimetrische bepaling van de standaarden. Bepaal aan de hand van de ijklijn de concentratie
van een onbekende Fe2+-oplossing.

2. Principe




Figuur 1 Kleurencirkel met bijhorende golflengtes



Wet van Lambert-Beer: E=ε . l. C
met: E = Extinctie

ε = extinctiecoëfficiënt [L.mol-1.cm-1]

l = weglengte [cm]

C = concentratie [mol.L-1]

Geldigheid van de Wet van Lambert-Beer:

 0,100 < E < 1,500
 Monochromatisch licht
 Verdunde oplossingen (C < 0,01 M)

VC-regel: V . C = V’ . C’

met: C = begin concentratie [ppm]

V = volume volpipet [mL]

C’ = verdunde-/eindconentratie [ppm]

V’ = volume maatkolf [mL]

, 3. Materiaal

glaswerk: chemicaliën:
- volpipet 10,00 mL - Standaard Fe3+-opl. (C = 100,0 ppm)
- volpipet 15,00 mL - 1,10-fenantroline . H2O (C = 0,1m/V% in water)
- volpipet 25,00 mL - hydroxylammoniumchloride-opl. (C = 5 m/V% in water)
- 8x maatkolf 50,00 mL - natriumacetaat-opl. (C = 10m/V% in water)
- 3x maatkolf 100,0 mL
Toestellen:
- Spectrometer (Thermo
Spectronic Genesys 20
Spectrophotometer 4001/4)


4. Bereiding ijkoplossingen

Eindverdunning:

Vpippet/mL C1/ppm Vmaatkolf/mL Ceind/ppm VF
10,00 25,00 50,00 5,000 5
10,00 20,00 50,00 4,000 5
10,00 15,00 50,00 3,000 5
10,00 10,00 50,00 2,000 5
10,00 X1 50,00 CX1 5
10,00 X2 50,00 CX2 5
+ 5mL orthofenantroline-opl. + 2mL hydroxylamine opl. + acetaatoplossing (tot pH = 5)

→ aanlengen met gedeïoniseerd water

Onbekende ijzeroplossing



Tussenverdunning:

Vpippet/mL Cstock/ppm Vmaatkolf/mL C1/ppm VF
25,00 100,0 100,0 25,00 4
10,00 100,0 50,00 20,00 5
15,00 100,0 100,0 15,00 6,7
10,00 100,0 100,0 10,00 10
→ aanlengen met gedeïoniseerd water



Voor de bereiding van de tussenverdunning en de eindverdunning moet er nauwkeurig gepipetteerd
worden zodat de ijkoplossing een nauwkeurige concentratie heeft. Ze moeten ook correct
aangelengd worden met gedeïoniseerd water.

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller Chemist123. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $9.80. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

62890 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$9.80  2x  sold
  • (0)
  Add to cart