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Verslag Spectrofotometrie - Fe2+

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Essay van 5 pagina's voor het vak Labportofolio aan de Artesis (resultaat: 14,5/20)

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  • December 1, 2021
  • 5
  • 2020/2021
  • Essay
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Verslaggevers: Groep:
Lector:
Labportfolio: algemene chemie 2
Beoordeling: 14,5/20

Colorimetrie: spectrofotometrie Fe2+
1. Doel

lmax bepalen aan de hand van een extinctiecurve. Stel bij deze golflengte de ijklijn op aan de hand van
een colorimetrische bepaling van de standaarden. Bepaal aan de hand van de ijklijn de concentratie
van een onbekende Fe2+-oplossing.

2. Principe




Figuur 1 Kleurencirkel met bijhorende golflengtes



Wet van Lambert-Beer: E=ε . l. C
met: E = Extinctie

ε = extinctiecoëfficiënt [L.mol-1.cm-1]

l = weglengte [cm]

C = concentratie [mol.L-1]

Geldigheid van de Wet van Lambert-Beer:

 0,100 < E < 1,500
 Monochromatisch licht
 Verdunde oplossingen (C < 0,01 M)

VC-regel: V . C = V’ . C’

met: C = begin concentratie [ppm]

V = volume volpipet [mL]

C’ = verdunde-/eindconentratie [ppm]

V’ = volume maatkolf [mL]

, 3. Materiaal

glaswerk: chemicaliën:
- volpipet 10,00 mL - Standaard Fe3+-opl. (C = 100,0 ppm)
- volpipet 15,00 mL - 1,10-fenantroline . H2O (C = 0,1m/V% in water)
- volpipet 25,00 mL - hydroxylammoniumchloride-opl. (C = 5 m/V% in water)
- 8x maatkolf 50,00 mL - natriumacetaat-opl. (C = 10m/V% in water)
- 3x maatkolf 100,0 mL
Toestellen:
- Spectrometer (Thermo
Spectronic Genesys 20
Spectrophotometer 4001/4)


4. Bereiding ijkoplossingen

Eindverdunning:

Vpippet/mL C1/ppm Vmaatkolf/mL Ceind/ppm VF
10,00 25,00 50,00 5,000 5
10,00 20,00 50,00 4,000 5
10,00 15,00 50,00 3,000 5
10,00 10,00 50,00 2,000 5
10,00 X1 50,00 CX1 5
10,00 X2 50,00 CX2 5
+ 5mL orthofenantroline-opl. + 2mL hydroxylamine opl. + acetaatoplossing (tot pH = 5)

→ aanlengen met gedeïoniseerd water

Onbekende ijzeroplossing



Tussenverdunning:

Vpippet/mL Cstock/ppm Vmaatkolf/mL C1/ppm VF
25,00 100,0 100,0 25,00 4
10,00 100,0 50,00 20,00 5
15,00 100,0 100,0 15,00 6,7
10,00 100,0 100,0 10,00 10
→ aanlengen met gedeïoniseerd water



Voor de bereiding van de tussenverdunning en de eindverdunning moet er nauwkeurig gepipetteerd
worden zodat de ijkoplossing een nauwkeurige concentratie heeft. Ze moeten ook correct
aangelengd worden met gedeïoniseerd water.

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