lmax bepalen aan de hand van een extinctiecurve. Stel bij deze golflengte de ijklijn op aan de hand van
een colorimetrische bepaling van de standaarden. Bepaal aan de hand van de ijklijn de concentratie
van een onbekende Fe2+-oplossing.
2. Principe
Figuur 1 Kleurencirkel met bijhorende golflengtes
Wet van Lambert-Beer: E=ε . l. C
met: E = Extinctie
ε = extinctiecoëfficiënt [L.mol-1.cm-1]
l = weglengte [cm]
C = concentratie [mol.L-1]
Geldigheid van de Wet van Lambert-Beer:
0,100 < E < 1,500
Monochromatisch licht
Verdunde oplossingen (C < 0,01 M)
VC-regel: V . C = V’ . C’
met: C = begin concentratie [ppm]
V = volume volpipet [mL]
C’ = verdunde-/eindconentratie [ppm]
V’ = volume maatkolf [mL]
, 3. Materiaal
glaswerk: chemicaliën:
- volpipet 10,00 mL - Standaard Fe3+-opl. (C = 100,0 ppm)
- volpipet 15,00 mL - 1,10-fenantroline . H2O (C = 0,1m/V% in water)
- volpipet 25,00 mL - hydroxylammoniumchloride-opl. (C = 5 m/V% in water)
- 8x maatkolf 50,00 mL - natriumacetaat-opl. (C = 10m/V% in water)
- 3x maatkolf 100,0 mL
Toestellen:
- Spectrometer (Thermo
Spectronic Genesys 20
Spectrophotometer 4001/4)
Voor de bereiding van de tussenverdunning en de eindverdunning moet er nauwkeurig gepipetteerd
worden zodat de ijkoplossing een nauwkeurige concentratie heeft. Ze moeten ook correct
aangelengd worden met gedeïoniseerd water.
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