100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
Summary Basic Introductory Histology Notes $2.81
Add to cart
Quickly navigate to
Preview
  2.  
  3. University Of The Witwatersrand (wits)
  4.  
  5. Anatomy (ANAT2021)

Summary

Summary Basic Introductory Histology Notes

 1 purchase
  • Course
  • Anatomy (ANAT2021)
  • Institution
  • University Of The Witwatersrand (wits)
  • Book
  • Histology

A detailed introduction to provide a foundation into understanding histology and histological components. These notes are a basic summary of histology to grasp the fundamental key elements of this topic.

Preview 3 out of 19  pages

Document information

  • No
  • Chapter 1 to chapter 3
  • December 2, 2021
  • 19
  • 2021/2022
  • Summary

Subjects

  • cell histology
  • histology
  • basic histology
  • cell components
  • anatomy
  • anat2021

Connected book

book image

Book Title:

Author(s):

  • Edition:
  • ISBN:
  • Edition:

Written for

  • University of the Witwatersrand (wits)
  • Anatomy (ANAT2021)
All documents for this subject (16)

Seller

avatar-seller
tiffanyjanewilton

Content preview

DEFINING HISTOLOGY

• Histology- Study of tissues and arrangement into organs
• Tissue = cells + extracellular matrix (ECM)
▪ Cells
– Major compartments = nucleus & cytoplasm(with organelles)
▪ ECM
– Structural network surrounding cells
– Influences cellular communication and function
– Contain fibres (collagen/elastic) and ground substance (proteoglycans, glycoproteins and
glycosaminoglycans)
• Structure is related to function!

HISTOLOGICAL TECHNIQUES

→ The routinely prepared hematoxylin and eosin–stained
section is the specimen most commonly studied.
• Haematoxylin
– Basic dye
– Stains acidic structures basophilic
– Colour: purple/dark blue
– Basic dyes react with anionic components of cells and tissue (components that carry a net negative
charge).
– A basic dye carries a net positive charge on its colored portion and is described by the general
formula [dye+ Cl− ].
– Anionic components include the phosphate groups of nucleic acids, the sulfate groups of
glycosaminoglycans, and the carboxyl groups of proteins.
– The ability of such anionic groups to react with a basic dye is called basophilia
– Hematoxylin is not, strictly speaking, a basic dye. It is used with a mordant (i.e., an intermediate link
between the tissue component and the dye).
– Tissue components that stain with hematoxylin also exhibit basophilia.
• Eosin
– Acidic dye
– Stains acidic structures eosinophilic
– Colour: pink
– An acidic dye carries a net negative charge on its colored portion and is described by the general
formula [Na+ dye−].
– Acidic dyes react with cationic groups in cells and tissues,
particularly with the ionized amino groups of proteins.
– The reaction of cationic groups with an acidic dye is
called acidophilia.
– Reactions of cell and tissue components with acidic
dyes are neither as specific nor as precise as reactions
with basic dyes

,→ A limited number of substances within cells and the extracellular matrix display basophilia.
• heterochromatin and nucleoli of the nucleus (chiefly because of ionized phosphate groups in nucleic
acids of both)
• cytoplasmic components such as the ergastoplasm (also because of ionized phosphate groups in
ribosomal RNA)
• extracellular materials such as the complex carbohydrates of the matrix of cartilage (because of
ionized sulfate groups).

→ Staining with acidic dyes is less specific, but more substances within cells and the extracellular matrix
exhibit acidophilia.
• most cytoplasmic filaments, especially those of muscle cells
• most intracellular membranous components and much of the otherwise unspecialized cytoplasm
• most extracellular fibers (primarily because of ionized amino groups).




→ Fixation, usually by a chemical or mixture of chemicals, permanently preserves the tissue structure for
subsequent treatments.
→ Specimens should be immersed in fixative immediately after they are removed from the body.
→ Fixation is used to:
• terminate cell metabolism,
• prevent enzymatic degradation of cells and tissues by autolysis (self-digestion),
• kill pathogenic microorganisms such as bacteria, fungi, and viruses
• harden the tissue as a result of either cross-linking or denaturing protein molecules.




Hematoxylin staining Eosin staining Hematoxylin & Eosin staining

, MICROSCOPY

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller tiffanyjanewilton. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $2.81. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

69052 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 15 years now

Start selling
$2.81  1x  sold
  • (0)
Add to cart
Added