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Samenvatting Formularium Farmacokinetiek
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Formularium biochemie 2e bachThermodynamiek
Gibbs vrije energie:
Δ G=Δ H −T∗Δ S
Δ G=ΔG0 −RTlnQ
Reactiequotiënt Q:
[ C ]∗[ D ]
Q=
[ A ]∗[ B ]
Evenwicht:
0
− ΔG
RT
K eq =e
0
−Δ G =RTln K eq
Enzymkineti ek
Reactiesnelheid voor de vorming van product:
v=k cat∗[ ES ]
Dissociatieconstante:
[ E ]∗[ S ]
k s=
[ ES ]
Vorming van ES = de dissociatie van het ES-complex + de ontbinding in enzym en product
k 1 [ E ] [ S ]=k−1 [ ES ] + k cat [ ES ]
Michaëlis Menten constante = de combinatie van al deze snelheidsconstanten
k −1 +k cat
K M=
k1
[ E ]∗[ S ]
K M=
[ ES ]
Michaëlis Menten vergelijking
V max∗[ S ]
v=
K M +[ S ]
Lineweaver burk plot:
1 Km 1
= +
v V max∗[ S ] V max
Hanes-woolf plot:
[S] [S] Km
= +
v V max V max
Eadie-hofstee plot:
v∗K M
v=V max −
[S]
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