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Samenvatting pathologisch bloedbeeld 2021
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samenvatting van de cursus pathologisch bloedbeeld
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Pathologisch bloedbeeldNORMALE HEMATOPOËSE
= erytropoëse zuurstoftransport
= megakaryopoëse hemostase (stollingscascade)
= granulopoëse neutrofielen (afweer), eosinofielen (parasitaire infecties), basofielen (mastcellen,
allergische reactie door histamine)
= monocytopoëse cellulaire en humorale respons
= lymfopoëse specifieke of adaptieve afweerrespons
INTRODUCTIE IN PATHOLOGISCH BLOEDBEELD
Diagnostiek
Stalen
Perifeer bloed
Beenmergaspiraat of beenmergbiopsie
Preparaten van kieren of weefsels
Technieken
Automaten voor automatische telling
o EDTA of lichaamsvochten
o 7 telkanalen
o Principes: colorimetrie, impedantie, lichtverstrooiing & fluorescentie, lichtverstrooiing &
conductiviteit
1
, o Hemoglobine (colorimetrie)
o RBC/PLT kanaal
Impedantie en hydrodynamische focussering
o Hematocriet
Obv MCV berekend (MCV * RBC)
o MPV (=mean platelet volume)
Halen uit PLT frequentiehistrogtam
Flowcytometrie
o Cellen door flow cell
o Verstrooiing
FSC: grootte
SSC: granules en kernvorm
o Fluorescentie
Celvolume (FSC)
Intracellulaire componenten (SSC)
Celactiviteit (DNA)
Cytoplasma-activiteit (RNA)
o WNR-kanaal
Scattergram: WBC, basofielen en NRCB
o WDF- kanaal
Lymfocyten, monocyten, neutrofielen en eosinofielen
o WPC-kanaal
Extra kanaal voor differentiatie van abnormale lymfo’s
Na flagging in WDF
o RET- kanaal
Reticulocyten onderscheiden van mature RBC
Low, medium, high fluorescences reticulocytes
2
, o PLT-F kanaal
Telling fluorescerende plaatjes
Fig. 1 WNR scattergram Fig. 2 WDF scattergram
Fig. 3 WPC scattergram Fig. 4 RET scattergram
3
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