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Assignment #2 on modules 4,5,6,7,8, 9
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Assignment #2 on modules 4,5,6,7,8, 9
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Assignment #2 on modules 4,5,6,7,8, 91. The purpose of PCR is to
- make many copies of an organism’s DNA sequence so that it can be analyzed further
2. After heating and cooling the mixture, which of the following synthesizes the
complementary strands of DNA?
- Taq polymerase
3. How many cycles of PCR give around 1000 copies of the DNA?
- 10
4. How many different types of ddNTPs are used in sequencing a strand of DNA in Sanger
sequencing?
- 4
5. At which stage of cell growth in a bioreactor is the growth the fastest?
- Exponential phase
6. The primer used in Sanger sequencing
- has a nucleotide sequence complementary to the 3' end of the region to be
copied
7. In Sanger sequencing, examining the electrophoresis gel and reading from bottom to top
(positive terminal to negative terminal), one base at a time, gives the sequence of DNA.
- True
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