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Nachweis der DNA-Methylierung in B. vulgaris undLängenbestimmung des 5S rRNA-Genarrys durch Fiber-
FISH
Praktikumsprotokoll zum Praktikum
Molekulare Cytokinetik
Eingereicht an der
Technischen Universität Dresden
Institut für Botanik
Lehrstuhl für Zell- und Molekularbiologie der Pflanzen
Prof. Dr. Stefan Wanke
Eingereicht bei:
Dr. Beatrice Weber
Eingereicht von: Natalie Schlüter
Matrikelnummer: 4930222
Dresden, 29.04.2021
, Inhaltsverzeichnis
Abbildungs- und Tabellenverzeichnis............................................................................................3
Abkürzungsverzeichnis.................................................................................................................4
1. Nachweis der DNA-Methylierung (Immunodetektion).............................................................5
1.1 Einleitung...........................................................................................................................5
1.2 Material und Methoden....................................................................................................6
1.3 Ergebnisse.........................................................................................................................6
1.3.1 Markierung der Sonden.............................................................................................6
1.3.2 Präparation von meiotischen Chromosomen............................................................6
1.3.3 Mikroskopische Auswertung der Immunodetektion.................................................7
1.4 Diskussion..........................................................................................................................8
2. Längenbestimmung des 5S-rRNA-Genarrays durch Fiber-FISH...............................................10
2.1 Einleitung.........................................................................................................................10
2.2 Material und Methoden..................................................................................................10
2.3 Ergebnisse.......................................................................................................................11
2.3.1 Markierung der Sonden...........................................................................................11
2.3.2. Präparation der Chromatin-Fiber............................................................................11
2.4 Diskussion........................................................................................................................13
3. Zusammenfassung..................................................................................................................14
Literaturverzeichnis....................................................................................................................15
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