Exam (elaborations)
BIOLOGY 171 BIO 171 / BIO171 MICRO BIO LAB EXAMS #1 - 8
- Course
- Institution
PORTAGE LEARNING BIOLOGY 171 BIO 171 / BIO171 MICRO BIO LAB EXAMS #1 - 8
[Show more]
Seller
Follow
Marilyn77
Reviews received
Content preview
PORTAGE LEARNINGBIOLOGY 171
BIO 171 / BIO171
MICRO BIO
LAB EXAMS #1 - 8
Lecture Exams
1. What three elements are used in an autoclave to sterilize equipment?
heat, pressure, and steam
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition?
125°C
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose
to best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer.
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes whereas hot air will
take several hours to achieve the same effect.
4. What type of incubator is pictured below?
Fixed incubator
,Answer the following questions.
1. At what temperature is the fixed incubator set to, as presented in the lab video?
37°C
2. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
3. What are the FOUR types of gloves presented in the lab video?
Latex, Nitrile, Thermal cold, Thermal heat
4. What THREE rules were discussed in regards to lab safety that would protect you and others from contamination?
1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
5. What are the main sections that should be found in a lab notebook? Name at least 4.
Objective, Procedure, Notes, Results and Deviations
Answer the following questions.
1. You are a lab instructor and Paul Smith has turned in his lab notebook for you to grade the lab experiment #2
on microscopy. Based upon what was covered in the lab video, how should Paul have entitled his lab experiment?
PS02 Microscopy
2. You arrive to your first day of work at a new lab. You are taking over for someone who took a new job at another lab.
Your boss informs you that because of time restraints, this person did not exactly follow the experimental protocol. In order
to proceed, you must know what he did differently. (1) According to the lab lecture, under what section of his notebook
would you look to find the experimental steps? (2) As changes to the experimental steps were made, what are these
differences called and how should it appear in the lab notebook?
, (1) Procedure—this is where the steps for the experimental protocol are recorded.
(2) Deviations. All deviations should be written in red to immediately bring attention to the changes in the protocol.
Answer the following questions
1. Identify the part of the microscope indicated by the arrow.
Neck (or arm)
2. Identify the part of the microscope indicated by the arrow.
Stage
3. Identify the part of the microscope indicated by the arrow.
fine focus
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Marilyn77. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $16.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
70840 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now