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Samenvatting extra definities

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vanuit de lessen de moeilijkste dingen

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  • February 1, 2022
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  • 2021/2022
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Wat gebeurt er tijdens:

rRNA processing:

45S pre rna => knippen => 41s rna

Its geknipt worden waardoor je 2 sequenties hebt: 1tje van 18s en 1 van 5,8 en 32seq hebt

Ets en its geknipt, mature rrna

mRNA processing:

(5’ cap additie), polyadenylering ,intronen weggeknipt

5’ en 3’ kant ingekort, intronen weg, aan 3’ cca seq toegevoegd => base gemodificeerd =>
alternatieve producten en apolaire structuren

tRNA processing:




functies ver RNA’s:

rrna: functionele eenheid ribosomen

mrna: eiwitsynthese

trna: staat mee in voor de synthese

secundaire structuur DNA:

dubbele helix gevormd, 2 dna strengen antiparallel lopen (5’ => 3’ en 3’ => 5’) baseparing volgens
watson en crick regels

transformerende capaciteit:

gladde stam in staat om de ruwe stam glad te maken, ruwe stam getransformeerd. Ook door proef
avery bevestigd: wat heeft invloed op capaciteit: dnase enige enzym effect op deze capaciteit van
bacteriën

oligonucleotide synthese:

5’ dntp groep: niet deelnemen aan heel proces van synthese, eest weghalen : deblokkeren

Vrije oh groep: geblokkeerde oligomolecule toevoegen en fosfotriesterbinding => alle
oligonucleotiden die niet gereageerd hebben weer geblokkeerd, stabiliseert en proces herhalen.

Voor u primer om genoeg bases te krijgen om als primer te kunnen gebruiken

ddNTP’s:

geen oh groep, gewoon h waardoor hier geen nieuwe base aan toegevoegd kan worden => eigenlijk
stop base



sanger sequencing:

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