Summary
Samenvatting Scheidingsmethoden, Cijfer: 9,2!
- Course
- Institution
Samenvatting van alles wat je met weten incl voorbeeldberekeningen. ik heb hier een 9,2 voor gehaald!
[Show more]
Seller
Follow
juliaesme
Reviews received
Content preview
Samenvatting scheidingsmethoden P4 jaar 1 LSC Inholland, 2020-2021Door Julia Muskee
Inhoudsopgave
Week 1: Inleiding en LLE.......................................................................................................................... 3
1. Extraheren ................................................................................................................................... 3
1.1 LLE........................................................................................................................................ 4
1.2 Samenvattend ........................................................................................................................... 7
Week 2: LLE en SPE.................................................................................................................................. 7
1. Vloeistof-vloeistof extractie ........................................................................................................ 7
1.1 pH effecten LLE .................................................................................................................... 8
2 SPE chromatografie ................................................................................................................... 11
2.1 Typen vloeistof-vaste stof chromatografie ............................................................................. 12
2.1 SPE ..................................................................................................................................... 12
2.2 De basis stappen van SPE .................................................................................................. 12
Week 3: Chromatografie ....................................................................................................................... 14
1. Chromatogram .......................................................................................................................... 14
1.2 GLC en LLC verdelingschromatografie ............................................................................... 15
1.3 LSC; Adsorptie.................................................................................................................... 15
1.4 Het Chromatogram ............................................................................................................ 16
1.5 Schotelgetal ....................................................................................................................... 20
Week 4: Van Deemter vergelijking ........................................................................................................ 22
1. Van Deemter vergelijking .......................................................................................................... 22
1.1 A-term; Eddy diffusie ......................................................................................................... 22
1.2 B-term; Diffusie in breedterichting ................................................................................... 23
1.3 C-term; stroomsnelheid door de kolom ............................................................................ 24
1.4 Van Deemter vergelijking in grafiek .................................................................................. 24
Week 5: Gaschromatografie.................................................................................................................. 25
1. Typen gaschromatografie.......................................................................................................... 25
2. Voordelen gaschromatografie en toepassingsgebied ............................................................... 26
3. Gaschromatograaf ..................................................................................................................... 26
3.1 Injectiepoort ............................................................................................................................ 26
3.2 Gaschromatograaf Colomn oven....................................................................................... 28
3.3 De oven.............................................................................................................................. 31
, 3.4 De kolom............................................................................................................................ 32
3.5 De detector ........................................................................................................................ 34
4. Dragergas................................................................................................................................... 35
Week 6: HPLC & Massaspectrofotometrie ............................................................................................ 36
5. Gaschromatografie .................................................................................................................... 37
6. High-Performance Liquid Chromatography HPLC ......................................................................... 37
6.1 Systeem HPLC .......................................................................................................................... 38
6.2 Scheiding in HPLC .................................................................................................................... 40
6.3 HPLC Detectie spectrofotometrie ........................................................................................... 43
6. Massaspectrootometrie ............................................................................................................ 44
6.1 opbouw MS ....................................................................................................................... 44
7. Samenvatting......................................................................................................................... 47
Week 7................................................................................................................................................... 48
8. Andere typen chromatografie ................................................................................................... 48
8.1 HPLC en deze chromatografie ........................................................................................... 50
9. Ion exchange chromatografie ............................................................................................... 51
9.1 Verschillende stationaire fasen ............................................................................................... 51
,Week 1: Inleiding en LLE
Leerdoelen:
- het begrip extractie, verdelingscoëfficiënt, extractierendement, en distributiecoëfficiënt te
beschrijven en de voorwaarden hiervan voor een extractie te noemen
1. Extraheren
Extractie: verschil in oplosbaarheid
LLE: vloeistof-vloeistof extractie
Basisbegrippen:
• Atoombinding / covalente binding
• Apolaire atoombinding
• Polaire atoombinding
• Vanderwaalskrachten
• Waterstofbruggen
Intermoleculaire krachten
• Waterstofbruggen; redelijk sterk (tot 10 kcal/mol)
• Vanderwaalskrachten / London dispersie krachten; zwak (0.5-2.5 kcal/mol); altijd aanwezig;
afhankelijk van molecuulmassa / ketenlengte en oppervlak / ruimtelijke structuur
• Dipool–dipool interactie; zwak (1 kcal/mol)
Waterstofbruggen:
, 1.1 LLE
2. Welke eisen stel je aan de oplosmiddelen?
Niet mengbaar: polariteitsverschil
3. Welk oplosmiddel is ‘onder’ in de scheitrechter?
De vloeistof met de grootste dichtheid
4. Hoe verdeelt een stof zich over beide oplosmiddelen?
Er ontstaat een verdelingsevenwicht
(partitioned between phases)
Kd = verdelingscoefficient =
Kow = partitie octanol / water
Log P = 10Kow KOW = verdeling stof tussen octanol en water. Van zeer veel moleculen is de log P
bekend
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller juliaesme. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $4.40. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
52510 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now