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Summary Dihybrid crosses

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Detailed notes on Dihybrid crosses

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  • February 21, 2022
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  • 2018/2019
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BLGY1232 Dihybrid crosses, independent assortment and multiple alleles

Dihybrid cross
 Used for monitoring 2 traits at the same time
 9:3:3:1 2 independent loci each represented by one dominant and one recessive
allele – independent assortment
 Number of segregating gene pairs (n)  number of phenotypic classes (2^n) 
number of genotypic classes (3^n)  total number of genotypes (4^n)
 Trihybrid cross looks at 3 independent traits at the same time
 Branch diagrams for numerous traits are easier
 Expected ratios in trihybrid cross for independent loci each represented by one
dominant and one recessive allele; 27:9:9:9:3:3:3:1
 Independent assortment – members of different gene pairs are assorted
independently of one another at gamete formation

Multiple genes controlling same trait
 When 2 parents create a different offspring which creates 4 different off spring in the
formation 9:3:3:1 there must be 2 genes segregating  the offspring of F1
phenotype depends on 2 alleles located at 2 independently assorting loci




 Allelism – different genes at the same locus
 Complementation - If loci are allelic you do not get wild type phenotypes when you
cross individuals homozygous for different recessive mutant alleles of the same gene
 if they are different loci wild type progeny result  if we cross 2 individuals who
mutate in he same gene you cant get a wildtype

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