Thesis
verslag Labo Farmaceutische analyse: Fotometrische bepaling van vitamine C
- Course
- Institution
Labo Farmaceutische analyse: Fotometrische bepaling van vitamine C + excel (labo verslag)
[Show more]
Seller
Followyilskasmet
Reviews received
Content preview
Naam: Yilska SmetKlas: 2FBTa
Datum uitvoering: 04/12/2020
Labo Farmaceutische analyse: Fotometrische bepaling van vitamine C
Doel:
Het doel is om de concentraties in µmol/l van de twee onbekende vitamine c-oplossingen te
berekenen. Hierbij wordt rekening gehouden met de verdunning. Om de concentraties te berekenen,
wordt er gebruik gemaakt van spectrofotometrie.
Principe: [1]
Vitamine C absorbeert licht bij 266 nm.
Absorbanties van de standaard oplossingen met een verschillende concentratie worden gemeten.
Met behulp van statistische verwerking worden vervolgens de concentraties van de onbekenden
berekend.
Gebruikte toestel:
- Analytische balans: Pioneer Ohaus M312
- Bovenweger: Sartorius Laboratory
- Droogstoof: Memmet M312-140
- Ultrasoon bad: VWR ultrasonic cleaner
- Fotospectrometer: Genesys 10S UV-Vis M312-602 Kwarts cuvets
Benodigde reagentia: [1]
Opmerking: Bij bepalingen in het UV-gebied mag geen aceton gebruikt worden om glaswerk te
drogen.
1. Buffer – pH 5,4 – KH2PO4 (0,03 mol/l) en Na2HPO4 (8,99.10-4 mol/l)
KH2PO4
Gegevens:
c= 0,03 mol/l
V= 3,0 l
M = 136,09 g/mol
mtheoretisch KH2PO4 = V * c * M
= 3,0 l * 0,03 mol/l * 136,09 g/mol
=12,25 g
= 1 * 10 g
mpraktisch KH2PO4 = 12,26 g
1
, Door mpraktisch kan cpraktisch worden berekend, zie afvalverwerking.
Na2HPO4.H2O (Na2HPO4 aanwezig)
m
n Na2HPO4.H2O =
M
0,48 g
= g
159,96
mol
= 0,0030 mol
MH2O = 18,00 g/mol
M Na2HPO4 = 159,96 g/mol – 18,00 g/mol
= 141,96 g/mol
m=n*M
= 0,0030 mol * 141,96 g/mol
= 0,43 g
Gegevens:
c= 8,99 * 10-4 mol/l
V= 3,0 l
M = 141,96g/mol
Met deze gegevens kan men de theoretische massa van Na 2HPO4 berekenen.
mtheoretisch Na2HPO4 = V * c * M
= 3,0 l * 8,99 * 10-4 mol/l* 141,96 g/mol
= 0,38 g
mpraktisch Na2HPO4 = 0,38 g
Door mpraktisch kan cpraktisch worden berekend, zie afvalverwerking.
- Bereiding;
Los de afgewogen massa’s van KH2PO4 en Na2HPO4 op in een grote erlenmeyer
Leng aan tot 3,0 l met Aqua Dest.
2. Natriumoxalaat oplossing – 0,0056 mol/l
Gegevens: c= 0,0056 mol/l
V= 3,0 l
M =134,00 g/mol
2
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller yilskasmet. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $6.95. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
52510 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now