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verslag Labo Farmaceutische analyse: Fotometrische bepaling van vitamine C
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Labo Farmaceutische analyse: Fotometrische bepaling van vitamine C + excel (labo verslag)
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Naam: Yilska SmetKlas: 2FBTa
Datum uitvoering: 04/12/2020
Labo Farmaceutische analyse: Fotometrische bepaling van vitamine C
Doel:
Het doel is om de concentraties in µmol/l van de twee onbekende vitamine c-oplossingen te
berekenen. Hierbij wordt rekening gehouden met de verdunning. Om de concentraties te berekenen,
wordt er gebruik gemaakt van spectrofotometrie.
Principe: [1]
Vitamine C absorbeert licht bij 266 nm.
Absorbanties van de standaard oplossingen met een verschillende concentratie worden gemeten.
Met behulp van statistische verwerking worden vervolgens de concentraties van de onbekenden
berekend.
Gebruikte toestel:
- Analytische balans: Pioneer Ohaus M312
- Bovenweger: Sartorius Laboratory
- Droogstoof: Memmet M312-140
- Ultrasoon bad: VWR ultrasonic cleaner
- Fotospectrometer: Genesys 10S UV-Vis M312-602 Kwarts cuvets
Benodigde reagentia: [1]
Opmerking: Bij bepalingen in het UV-gebied mag geen aceton gebruikt worden om glaswerk te
drogen.
1. Buffer – pH 5,4 – KH2PO4 (0,03 mol/l) en Na2HPO4 (8,99.10-4 mol/l)
KH2PO4
Gegevens:
c= 0,03 mol/l
V= 3,0 l
M = 136,09 g/mol
mtheoretisch KH2PO4 = V * c * M
= 3,0 l * 0,03 mol/l * 136,09 g/mol
=12,25 g
= 1 * 10 g
mpraktisch KH2PO4 = 12,26 g
1
, Door mpraktisch kan cpraktisch worden berekend, zie afvalverwerking.
Na2HPO4.H2O (Na2HPO4 aanwezig)
m
n Na2HPO4.H2O =
M
0,48 g
= g
159,96
mol
= 0,0030 mol
MH2O = 18,00 g/mol
M Na2HPO4 = 159,96 g/mol – 18,00 g/mol
= 141,96 g/mol
m=n*M
= 0,0030 mol * 141,96 g/mol
= 0,43 g
Gegevens:
c= 8,99 * 10-4 mol/l
V= 3,0 l
M = 141,96g/mol
Met deze gegevens kan men de theoretische massa van Na 2HPO4 berekenen.
mtheoretisch Na2HPO4 = V * c * M
= 3,0 l * 8,99 * 10-4 mol/l* 141,96 g/mol
= 0,38 g
mpraktisch Na2HPO4 = 0,38 g
Door mpraktisch kan cpraktisch worden berekend, zie afvalverwerking.
- Bereiding;
Los de afgewogen massa’s van KH2PO4 en Na2HPO4 op in een grote erlenmeyer
Leng aan tot 3,0 l met Aqua Dest.
2. Natriumoxalaat oplossing – 0,0056 mol/l
Gegevens: c= 0,0056 mol/l
V= 3,0 l
M =134,00 g/mol
2
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