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Instrumentele analyse 2Analysemethoden 2
bachelor Biomedische Laboratoriumtechnologie
afstudeerrichting Medisch Laboratoriumtechnologie
academiejaar 2017 - 2018
jaar 2 semester 1
Lector :Kathy Reyniers
kathy.reyniers@ap.be
T +32 3 220 55 80
IC92212207
,Instrumentele analyse 2
Analysemethoden 2
bachelor Biomedische Laboratoriumtechnologie
afstudeerrichting: Medische laboratoriumtechnologie
academiejaar 2013-2014
semester 1
Mevr.: Kathy Reyniers
kathy.reyniers@ap.be
T +32 3 220 55 80
,Inhoud
1 EMISSIEMETINGEN 3
1.1 FLUORESCENTIESPECTROMETRIE 5
1.1.1 Fluorescentie 5
1.1.2 Apparatuur 6
1.1.3 Ijklijn 6
1.1.4 Toepassing 7
1.2 NEFELOMETRIE 8
1.3 AES / VLAMFOTOMETRIE 9
1.3.1 Atoom en atoomspectroscopie 9
1.3.2 Apparatuur 12
1.3.3 Ijklijn 18
1.3.4 Toepassing 20
3 CHROMATOGRAFIE voor gevorderden 21
HPLC 22
3.1.1 Inleiding 22
Principe 22
3.1.3 Apparatuur 24
3.1.4 Studie van het chromatogram 39
3.1.5 Toepassing 39
3.2 HPLC-MS 41
3.2.1 Principe MS 41
3.2.2 Apparatuur 42
4 ELEKTROFORESE 43
4.1 ELEKTROFOCUSING 44
4.1.1 Basisprincipe 44
4.1.2 Dunlaag elektrofocusing 50
5 IONEN METEN 56
5.1 TYPE B: MEMBRAANELEKTRODEN 57
5.1.1 Inleiding 57
5.1.2 Glasmembraanelektroden 57
5.1.3 "solid-state" membraan 60
© AP Hogeschool – Instrumentele analyse 2 p
, 5.1.4 Vloeistofmembraan- elektroden 62
5.1.5 Gasgevoelige elektroden 64
5.1.6 Enzyme elektroden 65
5.1.7 Algemene opmerkingen bij meting met ion selectieve elektroden 66
5.2 POTENTIOMETRISCHE BEPALING 67
5.2.1 Statische bepaling 67
6 BEPALINGSMETHODEN 73
6.1 IJKLIJN methode 73
6.2 STANDAARDADDITIE methode 73
6.3 INTERNE STANDAARD methode 74
6.4 OEFENINGEN 75
© AP Hogeschool – Instrumentele analyse 2 p
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