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cursus instrumentele 2

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Cursus van Instrumentele analyse

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  • March 7, 2022
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Instrumentele analyse 2
Analysemethoden 2

bachelor Biomedische Laboratoriumtechnologie
afstudeerrichting Medisch Laboratoriumtechnologie

academiejaar 2017 - 2018
jaar 2 semester 1

Lector :Kathy Reyniers
kathy.reyniers@ap.be

T +32 3 220 55 80




IC92212207

,Instrumentele analyse 2
Analysemethoden 2

bachelor Biomedische Laboratoriumtechnologie
afstudeerrichting: Medische laboratoriumtechnologie
academiejaar 2013-2014
semester 1

Mevr.: Kathy Reyniers
kathy.reyniers@ap.be
T +32 3 220 55 80

,Inhoud

1 EMISSIEMETINGEN 3

1.1 FLUORESCENTIESPECTROMETRIE 5
1.1.1 Fluorescentie 5
1.1.2 Apparatuur 6
1.1.3 Ijklijn 6
1.1.4 Toepassing 7

1.2 NEFELOMETRIE 8

1.3 AES / VLAMFOTOMETRIE 9
1.3.1 Atoom en atoomspectroscopie 9
1.3.2 Apparatuur 12
1.3.3 Ijklijn 18
1.3.4 Toepassing 20


3 CHROMATOGRAFIE voor gevorderden 21

 HPLC 22
3.1.1 Inleiding 22
 Principe 22
3.1.3 Apparatuur 24
3.1.4 Studie van het chromatogram 39
3.1.5 Toepassing 39

3.2 HPLC-MS 41
3.2.1 Principe MS 41
3.2.2 Apparatuur 42


4 ELEKTROFORESE 43

4.1 ELEKTROFOCUSING 44
4.1.1 Basisprincipe 44
4.1.2 Dunlaag elektrofocusing 50


5 IONEN METEN 56

5.1 TYPE B: MEMBRAANELEKTRODEN 57
5.1.1 Inleiding 57
5.1.2 Glasmembraanelektroden 57
5.1.3 "solid-state" membraan 60



© AP Hogeschool – Instrumentele analyse 2 p

, 5.1.4 Vloeistofmembraan- elektroden 62
5.1.5 Gasgevoelige elektroden 64
5.1.6 Enzyme elektroden 65
5.1.7 Algemene opmerkingen bij meting met ion selectieve elektroden 66

5.2 POTENTIOMETRISCHE BEPALING 67
5.2.1 Statische bepaling 67


6 BEPALINGSMETHODEN 73

6.1 IJKLIJN methode 73

6.2 STANDAARDADDITIE methode 73

6.3 INTERNE STANDAARD methode 74

6.4 OEFENINGEN 75




© AP Hogeschool – Instrumentele analyse 2 p

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