Case
BIOS 225 Lab Report Week 2- Transferring Microbes While Using Aseptic Technique
- Course
- Institution
BIOS 225 Lab Report Week 2- Transferring Microbes While Using Aseptic Technique
[Show more]
Seller
Follow
10SALES
Reviews received
Content preview
BIOS 225 Lab Report Week 2- TransferringMicrobes While Using Aseptic Technique
Introduction:
Purpose: To learn an aseptic technique to reduce the minimum contamination, while
transferring bacteria from one place to another place by using inoculating loop or a needle, as
well as to isolate bacteria colony using the streak method.
Materials and Methods: Test tubes, bacteria (water), tops to test tubes, tube rack, LB agar
plates, media solution, Bunsen burner, and transfer loops. Safety equipment included goggles
and gloves.
Aseptic technique: a method designed to prevent contamination from microorganisms.
Results and Discussion: During this lab, the instructor explained how to use aseptic
techniques while transferring bacteria from one area to another. The instructor also
demonstrated how to transfer bacteria from tube to tube as well as from tube to agar plate using
aseptic technique. In order to practice aseptic technique and transfer bacteria, the following
steps must be followed.
Transferring from tube to tube steps using aseptic technique:
(a) Turn on Bunsen burner and sterilize loop over flame (until loop turns red).
(b) Take top off bacteria, warm opening of bacteria tube, then put loop in tube to get a
sample
(c) Place top back on to bacteria tube.
(d) Remove top from media tube, warm opening of media tube, then place bacteria
sample from the loop into media.
(e) Place top on media
(f) Use Bunsen burner to re-sterilize loop.
Transferring bacteria from tube to LB agar plate to conduct streak test using aseptic technique:
(a) Turn on Bunsen burner and sterilize loop over flame (until loop turns red).
(b) Take top off bacteria, warm opening of bacteria tube, then put loop in tube to get a
sample
(c) Place top back on to bacteria tube.
(d) Take top off LB agar plate and choose one side to do the first streak.
(e) Second streak, make sure to send loop through streak one.
(f) Third streak, make sure to send loop through streak two but not streak one.
(g) Fourth streak, make sure to send loop through streak three but do not touch first or
second streak. The fourth streak should be the most diluted streak.
(h) Place top on LB agar with streaks and put up to inoculate.
(i) Use Bunsen burner to re-sterilize loop.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller 10SALES. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $4.00. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
77333 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now