100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
Previously searched by you
VU Biomedical sciences Genetics experiment 1: Working with small volumes. & experiment 2: Diagnostic restriction digest of the human Hemoglobin gene. (year 1)$6.00
Add to cart
VU Biomedical sciences Genetics experiment 1: Working with small volumes. & experiment 2: Diagnostic restriction digest of the human Hemoglobin gene. (year 1)
8 views 0 purchase
Course
Genetics (AB_1135)
Institution
Vrije Universiteit Amsterdam (VU)
Book
ISE Genetics
Genetics experiment 1: Working with small volumes. & experiment 2: Diagnostic restriction digest of the human Hemoglobin gene Biomedical sciences year 1.
Summary ISE Genetics Analysis and Principles - Genetics Part 2!!
Chapter 1, overview of genetics
Complete Test Bank Genetics Analysis and Principles 7th Edition Brooker Questions & Answers with rationales (Chapter 1-29)
All for this textbook (60)
Written for
Vrije Universiteit Amsterdam (VU)
Bachelor Biomedical Sciences
Genetics (AB_1135)
All documents for this subject (37)
Seller
Follow
bram2001
Reviews received
Content preview
Wednesday, 22 september, 2021
Experiment 1: Working with small volumes
The aim of the experiment
The aim of the experiment is to learn how to use a (micro)pipette, because a (micro)pipette
is a very commonly used tool in the lab. During this experiment, we will learn how to use
three micropipettes, by practicing with three pipettes with different volume ranges. We will
also learn how to hold a pipette correctly and how to read the correct volume on the volume
indicator. After mastering the use of a (micro)pipette, we will need to make a series of
dilutions of the coloured compound; Methyl blue. The concentrations need to be maesured
afterwards, because we’ll need to plot the results in a graph. The graph will give us a good
representation of how accurate your pipetting is. The line fit of the points in the graph will
indicate how accurate our pipetting is. Thus, If your pipetting skills are good, the graph of
the amount of Methyl blue versus the absorbance in the spectrophotometer should give a
straight line.
Hypothesis
The graph of the amount of Methyl blue versus the absorbance in the spectrophotometer
should give a straight line if your pipetting skills are good. My hypothesis is that my pipetting
skills are good enough to produce a straight line in the graph. Therefore, I expect that my
line will be straight in the graph.
Material and Methods
(Material per person)
• A tube of Methyl blue solution (2mg/mL)
• 11 plastic cuvettes
• micropipette P20
• micropipette P200
• micropipette P1000
• micropipette tips (yellow and blue)
• water
• spectrophotometer
The given solution of Methyl blue is 2 miligrams per mililiter.
The given solution has a ratio of 2:1, so the converted solution of Methyl blue is:
2 micrograms per microliter (2 μg/μL).
Methyl blue volume Water volume (μL) Total volume (μL) μg of Methyl blue in
the cuvette
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller bram2001. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $6.00. You're not tied to anything after your purchase.
