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STRAIGHTERLINE BIO 250L Lab 3 Structure & Microscopy 2022

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1. What determines if a bacterial cell is Gram-positive or Gram-negative? The structure of a cell wall determines whether the cell is Gram-positive or Gramnegative The cell walls of Gram-positive bacteria are made up of thick layers of peptidoglycan. When Gram positive cells are treated to a Gram...

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  • April 14, 2022
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  • 2021/2022
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Lab 3 Structure & Microscopy BIO250L

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Pre-Lab Questions
1. What determines if a bacterial cell is Gram-positive or Gram-negative?

The structure of a cell wall determines whether the cell is Gram-positive or Gram-

negative The cell walls of Gram-positive bacteria are made up of thick layers of

peptidoglycan. When Gram positive cells are treated to a Gram stain technique, they

turn purple. Gram negative bacteria have a thin coat of peptidoglycan on their cell walls



2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research

and describe one other type of acellular organism. What characteristics about this organism

classify it as acellular?

Viroids are another sort of acellular creature. Viroids are the tiniest pathogens, consisting

only just a single strand of RNA and no protein covering. Viroids, like prions and viruses,

are categorised as acellular because they lack all of the necessary properties of a live

cell. Viroids can't proliferate without a host cell, and they can't survive for long without

one



3. Bacteria have many different shapes that often determine their class. Research and form a

hypothesis on the evolutionary reasons for so many different bacterial morphologies.

Bacteria, like other microbes, respond to their surroundings by growing and adapting.

After doing some study and thinking about why bacteria have so many various shapes

that distinguish which class they belong to, I came up with the following hypothesis:

Bacterial morphologies exist because an organism's shape changes throughout time to

suit the environment in which it lives

, Lab 3 Structure & Microscopy BIO250L

4. Do a search online or look in your textbook for 1-2 antibiotics that affect Gram-positive bacteria

and list them. On what part of the cell do the antibiotics usually work? List one or two antibiotics

that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be

sure to cite your sources in your answer.)

Penicillin and cloxacillin are antibiotics that attack gram-positive bacteria. Antibiotics

operate by destroying the bacteria's cell wall, according to an article on the Live Science

website by Joseph Castro. Amikacin and neomycin medicines are two antibiotics that

attack gram-negative bacteria. The outer membrane of the call is frequently attacked by

these antibiotics.



5. Why do you think it is important to identify a bacterial disease in a patient before prescribing any

antibiotic treatments? (Be specific.)

Click here to enter text.Each bacterial disease is different, and each antibiotic treatment

only attacks specific things with each bacterium so if a patient is prescribed an antibiotic

that attacks a structure that is healthy or not needed to be killed, then it can result in a

profoundly serious problem. For example, if a patient who has a disease caused by

gram-negative bacteria is prescribed penicillin, then the penicillin antibiotic will do its job,

but it will not be effective because it targets the coll wall of bacteria and destroys it by

bursting the call wall when the disease would be caused by cells that do not contain a

cell wall. In return that will not help the patient's disease but instead could cause even

more problems.



EXPERIMENT 1: Staining
Data Tables
Table 1: Experiment 1 Staining Observations

Violet Crystal

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