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Summary Microbiology EDUQAS A level $4.56   Add to cart

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Summary Microbiology EDUQAS A level

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Summary of EDUQAS BIOLOGY component 1: Microbiology

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  • May 23, 2022
  • 2
  • 2021/2022
  • Summary
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spherical ( coccus )
rod ( bacillus )
shape
spiral ( spirillum)


Gram positive
↳ thicker

↳ no
layer at

lipopolysaccharide
peptidoglycan
layer
] -




peptidoglycan

↳ crystal violet stain
peptidoglycan retains
violet stain
*
crystal
Gram negative
lipopolysaccharide
-




↳ thin layer of peptidoglycan
↳ has
lipopolysaccharide layer -


peptidoglycan
and
→ protect against penicillin lysozyme
* Safran in .
.




Condition for cell
growth
① nutrients :
glucose ,
nitrogen

④ water
③ temperature 125-4500
↳ 37°C mammalian
pathogens
↳ above 90°C ( thermophiles)

④ pH , optimum slightly alkaline

⑤ oxygen : -


obligate aerobe

-
facultative anaerobe

-


obligate anaerobes .




Aseptic technique
-
heat at 121°C for 15° in autoclave/ pressure cooker

-


benches → disinfect with 3% lysol
☒ incubate bacteria at 250C instead of 37°C

↳ pathogenic microorganisms .




Petri dishes should secured in
be with tape so
oxygen could get
-




* work wear Bunsen flame → air no microbes .

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