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Samenvatting klinische chemie 2: te kennen bepalingsreacties, normaalwaarden, formules, oorzaken verhoogd/verlaagd en overzicht enzymen op het einde$3.16
Samenvatting klinische chemie 2: te kennen bepalingsreacties, normaalwaarden, formules, oorzaken verhoogd/verlaagd en overzicht enzymen op het einde
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Course
Klinische Chemie 2
Institution
Hogeschool Gent (HoGent)
Slides, notities, cursus: te kennen bepalingsreacties, normaalwaarden, formules, oorzaken verhoogd/verlaagd en overzicht enzymen op einde met plaats synthese, iso-vormen, functies, ...
- Enzymatisch:
𝑔𝑙𝑢𝑡𝑎𝑚𝑎𝑎𝑡 𝐷𝐻
α-ketoglutaraat + NH4+ + NADH + H+ ↔ glutamaat + NAD+ + H2O
(340 nm)
o Extinctie daalt
o Blanco bepaling om vrij ammonium te corrigeren
- Elektrochemisch: ionselectieve elektrode (potentiometrie op volbloed)
NH4+ diffundeert doorheen selectieve membraan ISE waardoor de pH van
de oplossing wijzigt
Oorzaken verhoogd/verlaagd
Verhoogde ureumspiegels in het bloed:
- Eiwitrijke voeding
- Verhoging van afbraak van eiwitten:
o Koorts
o Brandwonden
o Malnutritie (afbraak lichaamseigen EW), vasten
o Neoplasie
- Verminderde uitscheiding:
o Uitdroging: meer terugresorptie van H2O en ureum (tubuli)
o Nierinsufficiëntie, nierziekten
o Nefrolithiase
o Tumoren genitale tractus
o Vergroting prostaat
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