Summary
LS8 Biotechnology Process design summary
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This is a handwritten summary for LS8 Biotechnology Process design
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, PROCESS DESIGN:
LECTURE 1
IDES OF FERMENTATION -
BIOREACTOR OPERATION
°
UNCONTROLLED FERMENTATION
"
DIFFICULT TO REPRODUCE ,
OUTCOME IS VARIABLE
(
log .
WINE , BEER ,
CHEESE , COMPOSTING ,
VINEGAR ,
BIOGAS FORMATION )
-
CONTROLLED FER MENTATIONS
:
REPRODUCIBLE AND LESS VARIATIONS (
eg
.
CITRIC ACID ,
BAKERS YEAST , PROTEIN PRODUCTION
) .
↳
BATCH ,
FED BATCH ,
CONTINUOUS
BATCH FERMENTATION
:
ALL MEDIUM INGREDIENTS PRESENT From
conrumud
BUILD INDUSTRIAL
produced SIMPLE
m UP PRODUCTS
START . .
OF .
a
SCALE
→
S =
SUBSTRATE
Xb =
Biomass
✓ L, @ WD =
VOLUME
B-ATCD CULTURE
"
SUBSTRATE CONCENTRATIONS
AFFECTS GROWTH YIELD AND GROWTH RATE .
YIELD =
CELL DENSITY =
NUMBER OF CELLS
1mL .
AMOUNT OF NUTRIENTS INCREASE
=
YIELD INCREASES
, FED -
BATCH FERMENTATION :
BATCH FERMENTATION WITH ( CONCENTRATED )
FEED .
HIGHER CELL DENSITY AND PRODUCT CDNCEN
-
o TRDTION . DUE TO SUBSTRATE LIMITATION ,
LESS IN
-
→
Just geneon FECIT @NS . WIDELY USED ON INDUSTRI > L SCALE .
CONTINUOUS FERMENTATION :
CONSTANT VOLUME
:
MATE OF ADDITION =
RATE OF REMOVAL
F- FEED =
Fout ( =
DILUTION RATE )
STEADY STATE
:
VOLUME CONSTANT
,
NUMBER OF CELLS
CONSTANT .
NUTRIENT CONCENTRATION CONSTANT
CONTINUOUS CULTURE :
GROWTH YIELD DEPENDS ON
SUBSTRATE CONCENTRATION .
GROWTH RATE DEPENDS ON DILU TON RATE
WASHOUT : Hod Dilution rate
( > the -1 . )
STEADY STATE :
CONSTANT HIGH BACTERIAL CONCENTRATION
↳ @ WTI -
NUTRIENTS AND KINETICS
[ IN RATIO
:
ELEMENTAL COMPOSITION A GENERAL
CELLULAR
OF MICROBIAL CELL -
Ca H 702N VARIES IN composition →
. WITH GROWTH CONDITIONS .
MOLS
✓ DRIES AMONG SPECIES ,
eg
.
Saccharomyces CereViriaeC3.7zH6.n@r.gs
No .co , So . a t P.o.ozsko.es 't
↳SURE THAT NOTHING IS MISSING .
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