100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Samenvatting lab biotechnologie $6.43
Add to cart

Summary

Samenvatting lab biotechnologie

 33 views  0 purchase
  • Course
  • Institution

Samenvatting van 3 pagina's voor het vak Lab biotechnologie aan de EhB (volledig)

Preview 1 out of 3  pages

  • June 15, 2022
  • 3
  • 2021/2022
  • Summary
avatar-seller
Lab biotech


1. Isolatie van DNA

1.1. Isolatie van DNA uit wangepitheelcellen


- DNA w geïsoleerd met commerciële Chargeswitch kit (magnetische isolatie)
o Cellyse
o DNA / RNA bindt aan magnetische bolletjes
o DNA w opgelost
- Er w altijd op ijs gewerkt
o DNA & RNA breekt af indien niet koel bewaard
- Invloed op de DNA opbrengst
o Gebruikte techniek
o Donor: # cellen die w uitgescheden = versch per persoon
o Type wattenstaafje
- Staal moet onmiddellijk w gebruikt of w bewaard op 4°C (max 2 weken)
o Wangepitheel bevat bact & nucleasen die DNA aantasten


1.2. Concentratiebepaling van DNA mbv een Nanospec

- Nanospec = spectrofotometer
o Meet de concentratie v DNA, RNA & proteïnen (kleine volumes) adhv
lichtabsorptie
- A260/A280 ratio: zuiverheid v DNA aantonen
o Zuiver staal: 1,8 – 2,0
o Hoe hoger de contaminatie, hoe lager A260/A280 ratio


2. Visualisatie van DNA: gelelektroforese
- DNA / RNA mol w gescheiden obv hun lengte
- Migratie nr anode (kathode -> anode)
- Gel = moleculaire zeef
o Kleine mol geraken verder dan grote mol
- Agarose
o Lineair
o Galactaan (lost op in water)
o Afkomstig uit algen
- TBE buffer
o Tris = organische base
o Boorzuur = brengt pH op 0,8
o EDTA = neutraliseert divalente ionen (Ca+, Mg+)

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller StudentEhbLL. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $6.43. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

50843 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$6.43
  • (0)
Add to cart
Added