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Samenvatting methoden in biomedisch onderzoek hoofdstuk 10: chromatografie
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Ik was geslaagd voor alle vakken via mijn samenvattingen ;)
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Chromatografie (10)Methoden in biomedisch onderzoek
Mengsel van stoffen wordt opgelost in mobiele fase en terwijl die in beweging is, wordt die
in contact gebracht met de stationaire fase
Sterke interactie met stationaire fase trager
Minder interactie met stationaire fase sneller
Papier / dunne laag chromatografie
Elutie principe = eluens lost componenten op en voert ze mee door stationaire fase
Elutiesnelheid hangt af van
- Oplosbaarheid van component in eluens
- Adsorptie van component aan stationaire fase
- Adsorptie van eluens aan stationaire fase
Mobiele fase = solvent waarin componenten oplossen
Stationaire fase = papier / dunne laag met kleine korrels op glasplaat of kunststofplaat
Retentiefactor = vertragingsfactor = A/B
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