BIOD 171 Microbiology lab exams 1-9 LAB 1 Answer the following questions 1. What three elements are used in an autoclave to sterilize equipment? heat, pressure, and steam 2. What is th e minimum temperature an autoclave must be set at to achieve sterile condition? 125°C 3. If you are working in a ...
3 what pathogen is best identified using a smac agar
5 if you were required to grow 4 types of bacteria on a single agar plate without cross contaminating the samples
what method would you use
1
Written for
Portage Learning
BIOD 171
All documents for this subject (474)
Seller
Follow
ProfGoodlucK
Reviews received
Content preview
BIOD 171 Microbiology lab exams 1-9
LAB 1
Answer the following questions
1. What three elements are used in an autoclave to sterilize equipment?
heat, pressure, and steam
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition?
125°C
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time,
which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you
chose your answer.
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes
whereas hot air will take several hours to achieve the same effect.
4. What type of incubator is pictured below?
Fixed incubator
Answer Key
,Answer the following questions.
1. At what temperature is the fixed incubator set to, as presented in the lab video?
37°C
2. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
3. What are the FOUR types of gloves presented in the lab video?
Latex, Nitrile, Thermal cold, Thermal heat
4. What THREE rules were discussed in regards to lab safety that would protect you and others from
contamination?
1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
5. What are the main sections that should be found in a lab notebook? Name at least 4.
Objective, Procedure, Notes, Results and Deviations
Answer the following questions.
1. You are a lab instructor and Sally Miller has turned in her lab notebook for you to grade the lab
experiment #1 on lab safety. Based upon what was covered in the lab video, how should Sally have
entitled her experiment?
SM01 Lab Safety
2. You arrive to your first day of work at a new lab. You are taking over for someone who took a new
job at another lab. Your boss informs you that because of time restraints, this person did not exactly
follow the experimental protocol. In order to proceed, you must know what he did differently. (1)
According to the lab lecture, under what section of his notebook would you look to find the
, experimental steps? (2) As changes to the experimental steps were made, what are these
differences called and how should it appear in the lab notebook?
(1) Procedure—this is where the steps for the experimental protocol are recorded.
(2) Deviations. All deviations should be written in red to immediately bring attention to the
changes in the protocol.
LAB 2
1. Identify the part of the microscope indicated by the arrow.
Oculars (or eyepiece)
2. Identify the part of the microscope indicated by the arrow.
Objectives (or objective lens)
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller ProfGoodlucK. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.99. You're not tied to anything after your purchase.
