Samenvatting Moleculaire technieken leerjaar 2 eerste blok
26 views 1 purchase
Course
Moleculaire Technieken
Institution
Hogeschool Arnhem En Nijmegen (HAN)
Deze samenvatting van moleculaire technieken gaat voornamelijk over het onderwerp PCR, waarbij de hele PCR stappen worden uitgelegd met de daarbij horende componenten en mixen en waarom je iets wel/niet gebruikt + de cyclus, daarbij komen ook de kloneringsfactoren, regulatiefactoren, genoom editin...
uitgebreide samenvatting met ondersteuning van plaatjes
Written for
Hogeschool Arnhem en Nijmegen (HAN)
Biologie En Medisch Laboratoriumonderzoek
Moleculaire Technieken
All documents for this subject (4)
Seller
Follow
iris70
Reviews received
Content preview
Moleculaire technieken
-Waarom genen klonen:
>Heel veel kopieën maken van een gen
-PCR:
>DNA amplificeren
>Restrictie sites aan DNA zetten
-
-
-
-
Made by: Iris Gülcher
,-De Annealing temperatuur bepaal je aan de hand van de smelttemperatuur
>Annealing Temperatuur = Tm (smelttemperatuur) – 5 graden Celcius
-A/T is 2 graden Celcius
-G/C is 4 graden Celcius
-
-
-De dNTPs bestaan uit dATPs (deoxyadenosine triphosphates), dCTPs (Deoxycytidine triphosphates),
dTTPs (deoxythymidine triphosphates) en dGTP (Deoxyguanosine triphosphates), de bouwblokken
van de nieuwe DNA strengen
-
-
-
Made by: Iris Gülcher
, -
-
-
-
>
-
Made by: Iris Gülcher
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller iris70. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $6.10. You're not tied to anything after your purchase.
