Samenvatting Moleculaire technieken leerjaar 2 eerste blok
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Course
Moleculaire Technieken
Institution
Hogeschool Arnhem En Nijmegen (HAN)
Deze samenvatting van moleculaire technieken gaat voornamelijk over het onderwerp PCR, waarbij de hele PCR stappen worden uitgelegd met de daarbij horende componenten en mixen en waarom je iets wel/niet gebruikt + de cyclus, daarbij komen ook de kloneringsfactoren, regulatiefactoren, genoom editin...
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Written for
Hogeschool Arnhem en Nijmegen (HAN)
Biologie En Medisch Laboratoriumonderzoek
Moleculaire Technieken
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Content preview
Moleculaire technieken
-Waarom genen klonen:
>Heel veel kopieën maken van een gen
-PCR:
>DNA amplificeren
>Restrictie sites aan DNA zetten
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Made by: Iris Gülcher
,-De Annealing temperatuur bepaal je aan de hand van de smelttemperatuur
>Annealing Temperatuur = Tm (smelttemperatuur) – 5 graden Celcius
-A/T is 2 graden Celcius
-G/C is 4 graden Celcius
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-De dNTPs bestaan uit dATPs (deoxyadenosine triphosphates), dCTPs (Deoxycytidine triphosphates),
dTTPs (deoxythymidine triphosphates) en dGTP (Deoxyguanosine triphosphates), de bouwblokken
van de nieuwe DNA strengen
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Made by: Iris Gülcher
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Made by: Iris Gülcher
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