De theorie voor de praktijk immunologie samenvatting gaat over de achtergrondinformatie van de technieken die je in de praktijk gaat uitvoeren en die ook worden getoetst op het tentamen. Hierbij gaat het om de technieken die in de lessen worden gebruikt (ELISA, flowcytometrie, MTT en BD accuri C6)
-Overige aandachtspunten:
>Vloeistoffen/medium toevoegen bij 37℃
>Spullen desinfecteren met 70% ethanol, eigen handen met sterilium
>Denk na voordat je de flowkast in gaat
>Overzichtelijk ingedeelde werkruimte
>Cellen regelmatig onder de microscoop controleren
-Besmetting:
>Mogelijke bronnen:
*Waterbad
*CO2 incubator (meestal 5%)
*Onderzoeker
>Meest voorkomend:
*Bacteriën
*Schimmels/gist
>Hoe te herkennen:
*Slechte groei
*Troebel medium bij hechtende cellen
*Microscopisch vaststellen
*Bij twijfel, vraag je docent
-Cellen tellen:
>Telkamer vs automatische celteller
>Fuchs Rosenthal / Burker Turk
>Cellen/ml= (N cellen)/((N hokjes*b*l*d) )∗1000∗VF
>Voeg Trypan Blue toe 1:1
*Verdunningsfactor = 2
*Welke cellen tel je / wat doet Trypan Blue?
*Blauwe (dode) en kleurloze (levende) cellen
1
>Dus cellen/ml = N cellen x 10.000
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Made by: Iris Gülcher
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