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Basics of molecular biology computer practical answers
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Uitwerking van de eerste computer practical van het eerste jaar master Biomedical Sciences
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Computer Practical week 36:'Basics of Molecular Biology'
Perform the practical in groups of two
Name student 1: Cisca Vreeswijk
Name student 2: Valeria Teodosieva
1
, Molecular cloning in silico
This part of the course is meant to gain knowledge of DNA and protein sequences and to get
used to some example software that is commonly used in a molecular biology lab. For this
practical you’ll have to fill in this word document and one of two students in the team should
upload it on Canvas at the assignment ‘Upload Results for Computer Practical Week 36’. Name
the file [LastName_FirstName]_ [LastName_FirstName]_CP-wk36.doc
Using the Snapgene you will perform a number of assignments, like:
1. Finding open reading frames (ORFs)
2. Restriction enzyme analysis
3. In silico cloning
4. Primer design
5. Gateway cloning
Below you will find a short tutorial of the most common functions of Snapgne. Read it carefully,
as it will save you a lot of time.
The sequences you need are in the ”Computer Practicals week 36 and 37” module for this
course on Canvas. Download the file “PART II.zip” and extract the contents to a temporary
folder.
Introduction:
Open Snapgene, you will see this screen below
2
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