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Samenvatting Biology, ISBN: 9781292341637 Moleculaire Biologie
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Samenvatting Biology v Campbell hoofdstuk 7 Moleculaire Biologie.
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Summary of unit 8, the ecology of life - Biology, a global approach, 12th edition
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Samenvatting Biology chapter 19, 20, 21, 22, 23
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Samenvatting Biology chapter 13, 14, 15, 16
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tennisclips oofdstuk 7Microscopie
vergroting ratio tussen deechtegrootte en die van het beeld
resolutie scherpte minimum afstandtussen 2 punten die los van elkaar
gezien kunnen worden zonder 1 punt te worden
contrast zichtbare verschillen in helderheid tussen verschillende
structuren
Microscopen
brightfield helderveld microscoop ongekleurd kan je kleuren
fase contrast verschillen in dichtheid tonen verschillend contrast
differentieel interferentie contrast 3D weergave
fluorescentie microscopie gebruik van fluorescente markers
confocale microscopie gebruik laser ipv lamp onderdelen z n plakjes
deconvolutie meerdere plakjesmaken digitaal uit focus licht verw deren
Super resolutie microscopie individuele fluorescente moleculen
weergeven
elektronenmicroscopie
scanning EM oppervlakte weergeven
transmissie EM in plakjes k ken
, Celfractionering scheiden van onderdelen van de cel
1 Homogeniseren blenden
2
Differentieel centrifugeren steeds harder
scheiden op basis van grootte
kernen
mitochondria
microsomen membranen
ribosomen
Supernatant na centrifugeren hebje het neergeslagen materiaal
onderin de buis de vloeistof die erboven staat is de supernatant
Cellen
Alle cellen hebben
plasmamembraan
cytosol vloeistof in de cel zonder deorganellen
chromosomen
ribosomen
Prokaryote cellen
geen kern
DNA in een regio genaamd nucleoid
geenmembraan omgeven organellen
bevinden zich in het domein Bacteriën en Archaea
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