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Summary Unit 2- Practical Scientific Procedures and Techniques 2C
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Part three of the assignment for applied science BTEC distinction achieved references included
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Name Pasha AllamUNIT 2. Practical Scientific Procedures
and Techniques
Learning Aim C: Undertake chromatographic
techniques to identify components
in mixtures.
Title of Assignment Separate to Identify
Assessor Mr David Peacock
Issue date /
Submission deadline 01/04/2021
Vocational context
I am a newly appointed technical assistant at a large chemical plant, Chemcalequip. As
part of my induction period and to progress in this role, I have to demonstrate skills in the
use of different chromatographic techniques to separate and identify components in
mixtures.
I firstly need to explain the use of chromatographic techniques to separate mixtures.
I need to produce a report of the techniques used and analyse and evaluate the results
and suggest improvements.
The use of different chromatographic techniques to separate and identify
components in mixtures.
Paper chromatography- This technique called paper chromatography is the method of
placing a line of a sample solution onto chromatography paper. The chromatography paper
is placed into solvent water which is a shallow layer of liquid. The solvent will rise up the
paper once the sample mixture has met the solvent. Sometimes it is difficult to see The
Separation with one run and only one solvent. If this is the case a second run can be carried
out using different solvents in a different Direction on the chromatography paper. This
diagram below shows simple paper chromatography by using water as the solvent and ink
as the sample.
, Thin-layer chromatography (TLC)
This technique called the thin layer chromatography is usually popularly used in
Laboratories; it's similar to paper chromatography however instead of using paper which is a
stationary phase. thin-layer chromatography, however, uses a stationary phase of a
substance like silica gel or cellulose. thin-layer chromatography, when compared to paper
chromatography, has advantages because the solvent runs faster and there are better
separations. You can also choose different materials for different absorbances. the diagram
below shows in there chromatography and the thin layer plate could be silica gel for
example.
Mobile phase- the mobile phase is when the solvent travels through the paper and
carries different substances with it; this is one of the two phases which
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