Summary

Minor Medische Microbiologie Praktijk blok 1 samenvatting Voordeelbundel

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Course
Minor Medische Microbiologie Praktijk

Institution
Hogeschool Arnhem En Nijmegen (HAN)

In deze samenvatting komt heel uitgebreid de antibiotica resistentie en de gevoeligheidspatronen van de bacteriën aan bod. Deze samenvatting is gebaseerd op blok 1 van praktijk van de minor Medische Microbiologie. Er komt uitgebreid aan bod welke antibiotica resistenties er zijn en welke gevoeligh...

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Uploaded on October 14, 2022
Number of pages 70
Written in 2022/2023
Type Summary

nederlandstalig
voordeelbundel
antibioticaresistentie
gevoeligheidspatronen
flowcharts d
praktijk blok 1 medische microbiologie minor
bloedparasieten met overzicht
overige parasieten met overzicht

Institution
Hogeschool Arnhem en Nijmegen (HAN)
Education
Biologie En Medisch Laboratoriumonderzoek
Course
Minor Medische Microbiologie Praktijk

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Minor Medische Microbiologie praktijk blok 1 & 2

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1. Summary - Minor medische microbiologie praktijk blok 1 samenvatting voordeelbundel
2. Summary - Minor medische microbiologie praktijk blok 2
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Minor Medische
Microbiologie Praktijk

Made by: Iris Gülcher

,Inhoudsopgave
Macroscopische Morfologie .................................................................................................................... 5
Macroscopische morfologie bacteriën .................................................................................................. 10
Gramnegatieve coccen ...................................................................................................................... 10
Gramnegatieve staven ...................................................................................................................... 10
Gramnegatieve staafjes..................................................................................................................... 10
Enterobacteriaceae ........................................................................................................................... 11
Pseudomonadaceae .......................................................................................................................... 11
Staphylococcen.................................................................................................................................. 11
Staphylococcus aureus ...................................................................................................................... 12
Enterococcen ..................................................................................................................................... 12
Streptococcen.................................................................................................................................... 12
Contaminatie ..................................................................................................................................... 12
Grampositieve staafjes ...................................................................................................................... 13
Grampositieve staven (voor CAMP) .................................................................................................. 13
Gramkleuring ......................................................................................................................................... 14
Microscopische Morfologie ................................................................................................................... 14
Gramnegatieve staven ...................................................................................................................... 14
Grampositieve kokken ....................................................................................................................... 14
Gramnegatieve kokken ..................................................................................................................... 14
Gramnegatieve staafjes..................................................................................................................... 15
Grampositieve staven........................................................................................................................ 15
Katalase test .......................................................................................................................................... 15
Oxidase test (meestal gramnegatief) .................................................................................................... 15
Determinatie gramnegatieve staven ..................................................................................................... 17
Flowchart determinatietesten gramnegatieve staven ...................................................................... 17
Determinatietechnieken gramnegatieve staven ............................................................................... 18
Oxidase test ................................................................................................................................... 18
Pseudoscreen ................................................................................................................................ 18
API20E............................................................................................................................................ 18
Indolspottest ................................................................................................................................. 18
ß-glucuronidase ............................................................................................................................. 18
Determinatietechnieken Nonfermentors.......................................................................................... 19
Macroscopische/microscopische morfologie + oxidase test ........................................................ 19
API20NE test .................................................................................................................................. 19
Determinatie grampositieve kokken ..................................................................................................... 20

Made by: Iris Gülcher

, Flowchart determinatietesten grampositieve kokken ..................................................................... 20
Determinatietechnieken grampositieve kokken ............................................................................... 21
Katalase test .................................................................................................................................. 21
Staphaurex test ............................................................................................................................. 21
Buis coagulase test ........................................................................................................................ 21
Novobiocine test ........................................................................................................................... 22
Trehalose mannitol test ................................................................................................................ 22
Esculine test................................................................................................................................... 22
Esculine Telluriet test (indifferent Streptococcen) ....................................................................... 22
Na-desoxycholaat 10% (vergroenende Streptococcen) ................................................................ 23
Optochine test (vergroenende Streptococcen)............................................................................. 23
Prolex test (Hemolytische streptococcen) .................................................................................... 23
Determinatie gramnegatieve kokken .................................................................................................... 25
Flowchart determinatietesten negatieve kokken ............................................................................. 25
Determinatietechnieken gramnegatieve kokken .............................................................................. 26
Schuiftest ....................................................................................................................................... 26
Tributyrin ....................................................................................................................................... 26
Neisseria gonorrhoeae .................................................................................................................. 26
Determinatie gramnegatieve staafjes ................................................................................................... 28
Flowchart determinatietesten gramnegatieve staafjes .................................................................... 28
Determinatietechnieken gramnegatieve staafjes ............................................................................. 29
Satelliettest.................................................................................................................................... 29
Determinatie grampositieve staven ...................................................................................................... 31
Flowchart determinatietesten grampositieve staven ....................................................................... 31
Determinatietechnieken grampositieve staven ................................................................................ 32
Omgekeerde Campreactie (Clostridium perfringens) ................................................................... 32
Listeria monocytogenes ................................................................................................................ 33
Mengculturen scheiden van elkaar met behulp van platen.................................................................. 34
Antibiotica resistentie en gevoeligheidspatronen Bijzonder resistente micro-organismen (BRMO) ... 36
Hoofdgroepen β-lactam antibiotica .................................................................................................. 36
Gramnegatievie staven met resistentie = BRMO (bijzonder resistent micro organisme) ................ 37
Resistentie mechanisme ESBL (Extended Spectrum Beta-Lactamase) ......................................... 37
Resistentie mechanisme CPE (Carbapenemase-producerende Enterobacteriaceae) (vaak
pseudomonas) ............................................................................................................................... 38
Fluorochinolonen- en aminoglycosiden-resistentie (dubbelresistentie) ...................................... 38
AMP-C ............................................................................................................................................ 39

Made by: Iris Gülcher

, Effluxpomp .................................................................................................................................... 39
Grampositieve staven........................................................................................................................ 39
MRSA Meticilline-resistente Staphylococcus aureus .................................................................... 39
ERM-gen ........................................................................................................................................ 39
PENG1 ............................................................................................................................................ 39
Enterococcen ..................................................................................................................................... 40
VRE (ancomycine Resistente Enterokok) ...................................................................................... 40
Antibiotica groepen ........................................................................................................................... 40
Overzicht antibiotica ............................................................................................................................. 41
Bloedparasieten .................................................................................................................................... 43
Samenvatting van de bloedparasieten (Malaria) .............................................................................. 43
Overzicht de kenmerken van de 4 plasmodium soorten .................................................................. 47
Overige parasieten ................................................................................................................................ 48
Overzicht overige parasieten ............................................................................................................ 48
Parasitologische deremtinatie methodes ............................................................................................. 58
Bepaling parasietendichtheid bij Plasmodium falciparum ................................................................ 58
Ridley-concentratie methode............................................................................................................ 58
Elisa Giardia lamblia .......................................................................................................................... 58
Voorbereidende vragen + antwoorden praktijklessen ......................................................................... 59
Macroscopische morfologie .............................................................................................................. 59
Basistechnieken ................................................................................................................................. 59
Determinatie technieken................................................................................................................... 61
Katalase test ...................................................................................................................................... 62
Oxidase .............................................................................................................................................. 62
Gram negatieve staven...................................................................................................................... 63
Gram negatieve staafjes .................................................................................................................... 64
Gram positieve staven ....................................................................................................................... 64
Gram negatieve kokken..................................................................................................................... 65
Gram positieve kokken ...................................................................................................................... 65
Kleuringen ......................................................................................................................................... 67
Parasitologie kleuringen .................................................................................................................... 68
Technieken/methoden ...................................................................................................................... 69
Elisa Giardia lamblia .......................................................................................................................... 70

Made by: Iris Gülcher

, Macroscopische Morfologie

Bloedagarplaat MacConkey Grampreparaat Testen

1.1 -Grijze kolonies -Pigmentloze kolonies Gramnegatieve (slanke) Oxidase positief
-Middelgrote -Goed groeiend polymorfe staven Pseudomonas test =
kolonies -Rafelige kolonies (exogeen pigment
-Ruikt chemisch -Middelgrote kolonies groen) resistant dus
-Kartelig -Geen lactosefermentatie Pseudomonas
-Hemolytisch aeruginosa
1.2 - -Kleine kolonies (pinpoint) Gramnegatieve slanke Oxidase positief
Grijs/pigmentloze -Ronde kolonies polymorfe staven Pseudomonas test
-Kleine kolonies -Pigmentloze kolonies (Müller hinton agar +
(pinpoint?) -Bolle kolonies tablet) = resistant dus
-Ronde kolonies Pseudomonas
(sommige lijken aeruginosa
op
waterdruppels)
-Platte kolonies
-Glanzend

2.1 -Grijze kolonies -Ronde kolonies Gramnegatieve Oxidase negatief
-Middelgrote -Roze kolonies polymorfe staven API20E +
kolonies -Middelgrote kolonies bloedagarplaat of
-Platte kolonies -Openstaande kolonies indolspot en B-
-Geen strakke -Lactosefermentatie glucoronidase
rand Escherichia coli
-A-hemolytisch
2.2 -Grijze kolonies -Grote kolonies Gramnegatieve Oxidase negatief
-A-hemolytisch -Roze kolonies en sommige polymorfe staven API20E +
-Bolle kolonies kolonies nemen kleur van bloedagarplaat
-Grote kolonies medium op
-Glanzende -Glanzende kolonies
kolonies -Slijmerige kolonies
2.3 -Grijze kolonies -Kleine kolonies Gramnegatieve korte Oxidase negatief
-Losliggende -Bleke kolonie polymorfe staven API20E +
kolonies -Kleur van kolonie komt door bloedagarplaat
-Endogeen endogeen pigment -Serratia marcescens
pigment -Ronde kolonies
-Grote kolonies -Glanzende kolonies
-Ronde kolonies -Bolle kolonies
-Bolle kolonies
-Glanzende
kolonies
2.4 -Grote kolonies -Kleine kolonies Gramnegatieve lange Oxidase negatief
-Licht grijze -Geel medium staven API20E +
kolonies bloedagarplaat

Made by: Iris Gülcher

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