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Werkvoorschrift reductie van ethylacetoacetaat en immobilisatie
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Dit is een werkvoorschrift die gebruikt is voor het practicum Biokatalyse
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By: Genaside • 7 year ago
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Werkvoorschrift: Reductie van Ethylacetoacetaat en ImmobilisatieR. Reich
Werkvoorschrift:
Yeast reduction of Ethyl acetoacetate: (S)-(+)-Ethyl 3-Hydroxybutanoate
[Butanoic acid, 3-hydroxy-, ethyl ester, (S)]
Figuur 1: Reductie van Ethylacetoacetaat: (S)-(+)-Ethyl 3-hydroxybutanoaat
Week 1:
1. Aan een erlenmeyer van 1 L met 160 ml water wordt tijdens het roeren met een grote roervlo het
volgende toegevoegd:
- 30.0 g sucrose
- 20.0 g bakkersgist
2. Het mengsel wordt gedurende 1 uur bij ongeveer 30 °C geroerd. Let op 1 à 2 bubbels CO2 per
seconden.
3. Vers gedistilleerde ethylacetoacetaat wordt toegevoegd.
4. Voeg 2,0 g (0,0154 mol) ethylacetoacetaat toe en roer de fermentatie suspensie zolang mogelijke
bij kamertemperatuur.
5. Voeg aan het einde 2,0 g (0,0154 mol) ethylacetoacetaat toe.
6. Laat het mengsel gedurende 50 a 60 uur bij kamertemperatuur roeren.
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