ASCP MB Exam questions well solved graded A +

ASCP MB Exam questions well solved graded A +Acute Promylocity Leukemia (APL) t(15:17) PML / RARa genes Chronic Myelogenous Leukemia (CML) t(9:22) a.k.a. Philadelphia chromosome ABL1 / BCR genes Burkitt's non-Hodkin's Lymphoma t(8:14) dysregulation of c-myc gene breakpoints on ch.8 spread over 190kbp region 5' to 3' Follicular non-Hodkin's Lymphoma t(14:18) IgH / BCL2 genes Mantle Cell Lymphoma (MCL) t(11:14) CCND1 / IgH genes overexpression of cyclin D1 (cell cycle gene) MALT Lymphoma (a.k.a. Marginal Zone) t(11:18) API2 / MLT genes "mucosa-associated lymphoid tissue" Haploid Genome 3.3 billion bp Carbamazepine Resistance HLA B1502 Factor V Leiden c.1691GA / p.R506Q causes Thrombophilia Cystic Fibrosis mutation delta F508, 3bp deletion HERceptin Therapy HER2+/neu Overexpression of HER2 receptors causing cancer cells to grow Vancomycin Resistance (Enterococcus) van A MRSA (resistant staph) mec A Mycobacterium tuberculosis katG, inhA Most cancerous HPV Strain 16 - 70% of all cases Graft vs Host Disease Linked w CMV replication (Cytomegalovirus) Viral load change HCV .3log10 Viral load change HIV .5log10 HCV Genotypes Genotype 1 - 75% Genotype 2 and 3 - 20-25% DNA A260 50ug/ml/1OD (answer on ascp is .560) What inhibits topoisomerase? Irinotecan KCL in PCR Helps short DNA fragments range 100bp to 1000bp at 70mM to 100mM FISH Prade-Willi # of amplification products (1+E)^n RNA POL I rRNA RNA POL II mRNA, snRNA RNA POL III tRNA RNA POL IV mitochondrial RNA Histones Ionic; (+) charged Arginine and Lysine Cutaneous T-Cell Lymphoma (CTCL) Vorinostat - 1st FDA approved drug (also treats Sezary syndrome) HCV most virulent 1b HCV most conserved 5' UTR HLA want to PCR HLA-D beta exon 2 Invader Assay enzyme Cleavase Melting temp question 48 Why primer dimers 3' complementarity A260/A280 Protein 1.8-2.0 RNA Telomerase Reverse transcriptase Fragile X Syndrome 200 CGG repeats 5'UTR of chr.X Huntington's Disease 40 CAG repeats ex1 chr.4 Common oncogene K-Ras Protein involved in cell division Common tumor suppressor TP53 Leading DNA Pol Delta Lagging DNA Pol Alpha Repair DNA Pol Epsilon Mitochondrial DNA Pol Gamma Taq polymerase 5'-3' exonuclease bDNA enzyme alakaline phosphatase Separates DNA helicase Unwinds DNA topoisomerase Nephrotic kidney (Post-transplant virus) BK virus Kidney Failure HIV EBV - Epstein-Barr virus aka "Herpes" Post-transplant Lymphoproliferative patients Early diagnosis of EBV RT-qPCR homologous chismata formation prophase I Cell cycle entry G1 to S Hallmark feature of TNR disorders Anticipation [HLA Typing] Advantage of molecular testing over seriological phenotyping? 1. Viable cells are NOT needed 2. Null alleles can be detected