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ASCP MB: Extra-Practice Questions and correct answers 2022/2023
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ASCP MB: Extra-Practice Questions and correct answers 2022/2023The Klenow fragment of E. coli DNA polymerase I is used: to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? gel mobility shift assay ...

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  • November 21, 2022
  • 17
  • 2022/2023
  • Exam (elaborations)
  • Questions & answers

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  • ascp mb extra practice questions and correct answers 20222023
  • the klenow fragment of e coli dna polymerase i is used to label dna probes by random primer labeling
  • what is a solution hybridization

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ASCP MB: Extra-Practice Questions and
correct answers 2022/2023
The Klenow fragment of E. coli DNA polymerase I is used: - correct answersto label
DNA probes by random primer labeling.

What is a solution hybridization method for the detection of nucleic acid:protein
interactions? - correct answersgel mobility shift assay

_____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific
interaction occurs, visible with an apparent increase in molecular size on a gel due to
the protein:nucleic acid interaction. - correct answersGel mobility shift assay

Which probe is most often used to detect trisomy 21 by interphase FISH? - correct
answersalpha satellite DNA probe

_____ detect specific chromosomes and numbers of the specific chromosomes. -
correct answersAlpha satellite DNA probe

Which reagent generates a signal during the annealing stage of a quantitative real time
PCR reaction? - correct answersmolecular beacon

Methylation of cytosine residues is involved in the alteration of gene expression in a
number of cancers. Which sequencing technique is best suited to determine if
methylation of cytosine residues has taken place in the promoter region of a given
gene? - correct answersbisulfite sequencing

_____ is a modified chain terminator method specifically designed to detect methylated
nucleotides. - correct answersBisulfite sequencing

Which method is best suited to screen for mutations in a gene having multiple possible
mutations? - correct answerssingle strand conformational polymorphism (SSCP)

_____ is a screening test in which the exact nature of a mutation does not need to be
known in order to detect the presence of a mutation. - correct answersSingle strand
conformational polymorphism (SSCP)

What is the best method to rule out the possibility of genomic DNA giving a false-
positive result in an RT-PCR reaction? - correct answersuse of upstream and
downstream primers that span an exon-intron-exon region of the target

*amplicaons generated from genomic DNA would be larger than the specific amplicon
generated from a mature, spliced mRNA

,A common use for pulsed-field gel electrophoresis is: - correct answersepidemiological
typing of bacterial strains.

_____ after digestion of bacterial chromosomes with restriction enzymes is used to
determine similarity between bacterial isolates. - correct answersPFGE

A translocation in which chromosome pair creates a BCR/ABL1 fusion gene product
associated with CML?
a. 11;18
b. 14;18
c. 9;22
d. 9;14 - correct answerst(9;22)

The presence of a distinct band along with the smear indicates at least 1% of the
patient's B cells have a _____ of the Ig heavy chain. - correct answersmonoclonal
rearrangement

A molecular-based test commonly used in testing donors and recipients prior to organ
transplantation is: - correct answerssequence-specific PCR.

_____ is commonly performed to determine polymorphisms in class I and class II HLA-
DRB and HLA-DRQ genes. - correct answersSequence-specific PCR

DNA sequencing of hypervariable regions I and II is used to: - correct answersdetect
polymorphisms in human mitochondrial DNA.

HV1 and HV2 are sequenced to determine _____. - correct answersmitochondrial
inheritance.

Repeated discrepancies between patient identifiers will result in _____ - correct
answersthe error being documented to the laboratory supervisor and the risk manager
will conduct a root cause analysis.

An index of precision is statistically known as the: - correct answerscoefficient of
variation.

The _____ is the measure of relative random error expressed as a percentage. - correct
answerscoefficient of variation

The term used to describe reproducibility is: - correct answersprecision.

_____ is the degree to which results of multiple analyses of the same specimen agree. -
correct answersPrecision

, The process by which an agency or organization uses predetermined standards to
evaluate and recognize a program of study in an institution is called: - correct
answersaccreditation.

Using a common labeling system for hazardous material identification such as HMIS or
NFPA 704, which hazard is represented by:

the top red quadrant?
the right yellow quadrant?
the lower white quadrant?
the left blue quadrant? - correct answersflammability
reactivity
special reactivity
health

If the HMIS or NFPA 704 hazardous material identification system has a number 4 in
the left blue quadrant, it represents a: - correct answershigh health hazard

The ratings for the HMIS or NFPA 704 hazardous material identification systhem are 0-
4 with 0 being _____ and 4 being _____. - correct answersno hazard; most severe
hazard

Which chemical is a potential carcinogen? - correct answersformaldehyde

When hazardous chemicals are transferred from the original appropriately labeled
container(s) to a secondary container for immediate use by the person performing the
transfer, it: - correct answersdoes not require labeling.

A technologist spilled 10 gallons of formaldehyde on the floor. After determining the
chemical poses a significant health hazard, the first action step would be to: - correct
answersevacuate the area.

One of the elements of a written laboratory chemical hygiene plan is to: - correct
answersdesignate a laboratory chemical hygiene officer.

When working with sharp equipment and objects, use a: - correct answersmechanical
device.

Incident reports for occupational injury or illness should: - correct answersbe filed for all
incidents including near miss incidents.

According to OSHA, what type of warning sign should be posted in an area where an
immediate hazard exists and where special precautions are necessary? - correct
answersred, black and white "Danger" sign

All laboratory instruments should: - correct answersbe grounded or double insulated.

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