WGU Biochemistry PA| 96 QUESTIONS| WITH COMPLETE SOLUTIONS

What is the complementary sequence for the following DNA sequence? 5' - GCC ATC TCG AAT - 3' Correct Answer: 5' - ATT CGA GAT GGC - 3' Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four complete PCR cycles? Correct Answer: 16 What is the function of DNA polymerase in the process of PCR? Correct Answer: It recognizes the primers and uses the available dNTPs to replicate the template DNA sequence. What are possible blood types of the parents of a patient with blood type AB? Correct Answer: A and B Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is associated with chronic exposure to cocaine and contributes to drug addiction. What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to cocaine increases their expression? Correct Answer: The nucleosomes become more widely spaced. Mice with low levels of agouti gene expression tend to have yellow fur and are obese, while mice with higher levels of expression are brown and of normal weight. Studies show that expression of the agouti gene in mice can be controlled by epigenetics. What scenario describes epigenetic regulation of the agouti gene? Correct Answer: Mother mice fed methyl-rich diets during pregnancy give birth to offspring with decreased agouti gene expression compared to those fed a methyl-poor diet. Oxidative damage to DNA can often result in the alteration of a single nucleotide. Which DNA repair mechanism would repair this type of damage? Correct Answer: The mutation rate of all other genes is higher due to failure to repair. Which reaction is responsible for the formation of polypeptides? Correct Answer: Dehydration Which term describes the order of amino acids in a peptide chain? Correct Answer: Primary structure When using heat to denature a protein, which force is the first to be disrupted as temperature increases? Correct Answer: Hydrophobic interactions A commonly used technique for protein denaturation is adding an acid or a base to dramatically change the pH. Which force is most impacted by this change in pH? Correct Answer: Ionic bonds An active elderly patient is becoming frequentl