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ZSO 3 Bio informatica
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ZSO 3 - Uitgewerkte oefeningen van ZSO 3 van bioinformatica, biomedische wetenschappen UHasselt
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ZSO 3: VAN SEQUENTIE NAARFUNCTIE EN STRUCTUUR VAN
EEN EIWIT
ACHTERGROND
primaire structuur
wordt rechtstreeks op het eiwit bepaald door massaspectometrie of
edmandegradatie
op gen niveau door DNA of mRNA-sequencing
maar, eiwit is meer dan een sequentie => ook structuur (secundair, tertiair en quaternair) en
functie van het eiwit in de cel nodig
*eiwit is geen alleenstaand gegeven => begrijpen van interacties ook nodig om biologische
fenomenen te begrijpen
TOEPASSINGSVRAGEN
1. GA NAAR WEBSITE: HTTP://WWW.EXPASY.ORG/ EN DOE EEN QUERY IN DE
UNIPROTKB DATABANK MET HET 5-LETTERWOORD GAPDH
https://www.uniprot.org/uniprot/?query=gapdh&sort=score
HOEVEEL RESULTATEN LEVERT DE QUERY OP IN SWISS-PROT (REVIEWED) EN IN TREMBL
(UNREVIEWED)? SELECTEER ENKEL DE SWISS-PROT RESULTATEN, ZOEK HET HUMANE GAPDH
OP EN KLIK OP P04406. OP DEZE PAGINA IS EEN VIDEO BESCHIKBAAR (RECHTSBOVEN IN HET
SCHERM) DIE UITLEG VERSCHAFT OVER DE WEBPAGINA ZELF (OPTIONEEL).
https://www.uniprot.org/uniprot/P04406
ZOEK OP DEZE WEBPAGINA: DE ENTRY NAME, PRIMARY ACCESSION NUMBER, PROTEIN NAME,
GENE NAME, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCALIZATION (ENKEL DE
EXPERIMENTEEL BEPAALDE LOKATIES) , AANTAL ISOVORMEN, AANTAL AMINOZUREN DAT DE
CANONIEKE SEQUENTIE TELT ALSOOK HET OVEREENSTEMMEND MOLECULAIRE GEWICHT.
, entry name:
primary accession number
protein name and gene name
catalytic activity
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