Summary
Samenvatting medische bacteriologie 2
- Course
- Institution
Samenvatting van de cursus, slides en lessen van het vak medische bacteriologie 2.
[Show more]
Seller
Follow
juliemouton
Content preview
INHOUD1. Pre-analytische fase ........................................................................................................................................ 6
1.1. Wat is pre analytische fase? .................................................................................................................. 6
1.2. Soorten afnames ................................................................................................................................... 6
Type 1 .............................................................................................................................................................. 6
TYPE 2 .............................................................................................................................................................. 7
TYPE 3 .............................................................................................................................................................. 7
1.3. Soorten stalen........................................................................................................................................ 8
Bloed ............................................................................................................................................................... 8
Punctievloeistoffen – empyeem type 1 .......................................................................................................... 8
Lumbaal vocht ................................................................................................................................................. 8
Weefselmateriaal ............................................................................................................................................ 9
Etter, wondvocht, klierpunctievocht ............................................................................................................... 9
Keel- en neusspecimens .................................................................................................................................. 9
Onderste luchtwegen ...................................................................................................................................... 9
Urine type 2................................................................................................................................................... 10
Faeces type 3................................................................................................................................................. 10
1.4. Overzicht van de mogelijke tools voor staalafname en staalbewaring ............................................... 11
Wattendrager ................................................................................................................................................ 11
E-swab ........................................................................................................................................................... 11
Spuit .............................................................................................................................................................. 11
1.5. Steriele afname van versch soorten microbiologische stalen en begeleidende documenten ............ 11
1.6. Transport van de stalen ....................................................................................................................... 12
Transport-omstandigheden: ......................................................................................................................... 13
1.7. Organisatie van de pre analytische fase in het labo ............................................................................ 13
Ontvangst en registratie van het specimen in het Laboratory Information System ..................................... 13
Sorteren van de stalen .................................................................................................................................. 13
Sneltesten ..................................................................................................................................................... 13
Microscopie (= rechtsreeks onderzoek) ........................................................................................................ 14
Inzetten van de kweek .................................................................................................................................. 16
1.8. Kwaliteitszorg ...................................................................................................................................... 16
2. Gram negatieve kokken ................................................................................................................................ 17
2.1. Neisseria species .................................................................................................................................. 17
Morfologie..................................................................................................................................................... 17
Species .......................................................................................................................................................... 17
Pathogeen vermogen meningokok ............................................................................................................... 17
1
, Pathogeen vermogen bij de gonokok (gonorroe) ......................................................................................... 17
Laboratorium diagnosa ................................................................................................................................. 18
Serotypes en vaccinatie ................................................................................................................................ 18
Gevoeligheidsbepaling .................................................................................................................................. 18
2.2. Moracella catarrhalis ........................................................................................................................... 19
Pathogeen vermogen .................................................................................................................................... 19
Laboratorium diagnose ................................................................................................................................. 19
3. Gram positieve kokken.................................................................................................................................. 20
3.1. STaphylococcus aureus ........................................................................................................................ 20
Morfologie..................................................................................................................................................... 20
Pathogeen vermogen .................................................................................................................................... 20
Laboratoriumdiagnostiek .............................................................................................................................. 20
Antibiogram .................................................................................................................................................. 21
Soorten MRSA ............................................................................................................................................... 22
3.2. Coagulase negatieve Stafylokokken (CNS)........................................................................................... 22
Pathogeen vermogen .................................................................................................................................... 22
3.3. Streptokokken ..................................................................................................................................... 22
Indeling op basis van hemolysepatroon ....................................................................................................... 22
Streptococcus pyogenes ............................................................................................................................... 23
Streptococcus agalctiae ................................................................................................................................ 23
3.4. Groep D streptokokken ....................................................................................................................... 25
Pathogeen vermogen .................................................................................................................................... 25
Laboratoriumdiagnostiek .............................................................................................................................. 25
3.5. Viridans streptokokken ........................................................................................................................ 25
Pathogeen vermogen .................................................................................................................................... 25
Laboratorium diagnostiek ............................................................................................................................. 25
3.6. Streptococcus pneumoniae ................................................................................................................. 26
Pathogeen vermogen .................................................................................................................................... 26
4. Gram negatieve bacillen ............................................................................................................................... 27
4.1. Belangrijkste geslachten ...................................................................................................................... 27
4.2. Anaerobiose......................................................................................................................................... 27
4.3. Algemene kernmerken van ifnecties bij anaerobe kiemen ................................................................. 27
Biofilmvorming (examen) .............................................................................................................................. 27
4.4. Morfologie (gramkleuring) .................................................................................................................. 28
Porphyromonas/prevotella ........................................................................................................................... 28
5. Anaerobe grampositieve bacillen.................................................................................................................. 29
5.1. Soorten ............................................................................................................................................... 29
2
, 5.2. Clostridium species .............................................................................................................................. 29
Clostridium perfringens (soldaat) ................................................................................................................. 29
Clostridium botulinum (zwemmen,lopen, fietsen) ....................................................................................... 30
Clostridium tetani (tetanus) .......................................................................................................................... 30
Clostridium ramosum en septicum ............................................................................................................... 30
!! Clostridium difficile !! ............................................................................................................................... 30
5.3. Actinomyces ........................................................................................................................................ 31
5.4. Propionibacterium ............................................................................................................................... 31
6. Aerobe grampositieve staven ....................................................................................................................... 32
6.0. Bacillus species .................................................................................................................................... 32
Bacillus cereus ............................................................................................................................................... 32
Bacillus anthracis ........................................................................................................................................... 32
Laboratoriumdiagnostiek .............................................................................................................................. 32
Behandeling .................................................................................................................................................. 33
6.2. Corynebacterium species .................................................................................................................... 33
C. diphtheriae ................................................................................................................................................ 33
Andere corynebacteriën ............................................................................................................................... 33
Laboratoriumdiagnostiek .............................................................................................................................. 34
6.3. Erysipelothrix ....................................................................................................................................... 34
Laboratoriumdiagnostiek .............................................................................................................................. 34
6.4. Lactobaccilus ....................................................................................................................................... 34
Laboratoriumdiagnostiek .............................................................................................................................. 34
Gardnerella ....................................................................................................................................................... 35
Gardnerella vaginalis ..................................................................................................................................... 35
Diagnostiek.................................................................................................................................................... 35
6.5. Listeria ................................................................................................................................................. 36
Laboratoriumdiagnostiek ...................................................................................................................................... 36
6.6. Nocardia .............................................................................................................................................. 37
Pathogeen vermogen .................................................................................................................................... 37
Laboratoriumdiagnostiek .............................................................................................................................. 37
Behandeling .................................................................................................................................................. 37
6.7. Rhodococcus equi ................................................................................................................................ 38
6.8. A. haemolyticum .................................................................................................................................. 38
Vragen ............................................................................................................................................................... 38
7. Spirochaetales (speciale celwand!) .............................................................................................................. 39
Treponema Pallidum ......................................................................................................................................... 39
Laboratoriumdiagnostiek .............................................................................................................................. 40
3
, Treponema pallidum haemagglutination assay (TPHA) ................................................................................ 42
Fluorescent treponemal antibody absorption test (FTA-ABS) ...................................................................... 42
Venereal disease research laboratory test (VDRL) ........................................................................................ 42
Behandeling .................................................................................................................................................. 42
Borrelia burgdorferi .......................................................................................................................................... 42
8. Mycobacterium (speciale celwand!) ............................................................................................................. 43
8.1. Situering............................................................................................................................................... 43
8.2. Algemene kernmerken ........................................................................................................................ 43
Celwandstructuur .......................................................................................................................................... 43
8.3. Mycobacterium indeling ...................................................................................................................... 44
8.4. Pathogene myobacterium ................................................................................................................... 44
Myobacterium tuberculosis .......................................................................................................................... 44
Mycobacterium Leprae ................................................................................................................................. 45
8.5. Microscopisch onderzoek van myobacterium ..................................................................................... 45
Kweek: voorbehandeling............................................................................................................................... 45
Kweek: automaten ........................................................................................................................................ 45
IGRA: Interferon gamma release assay !!!!! .................................................................................................. 45
Behandeling .................................................................................................................................................. 45
9. Mycoplasma en Chlamydia (speciale celwand) ............................................................................................. 46
9.1. Mycoplasmata ............................................................................................................................................ 46
Mycoplasma pneumoniae ............................................................................................................................. 46
M. hominis, M. genitalium& Ureaplasma urealyticum ................................................................................. 46
Kleinste prokaryoten die kunnen groeien op bodems .................................................................................. 47
Diagnostiek.................................................................................................................................................... 47
9.2. Chlamydiae .......................................................................................................................................... 47
unieke groeicyclus ......................................................................................................................................... 47
Belangrijke ziekteverwekkers ........................................................................................................................ 48
Diagnostiek.................................................................................................................................................... 48
10. Keuze van cultuurbodems voor diverse specimens ................................................................................. 49
10.2. Neus – keel – oren ............................................................................................................................... 49
Commensale microbiota ............................................................................................................................... 49
Acute keelontsteking..................................................................................................................................... 49
Pathogenen hogere luchtwegen: keel .......................................................................................................... 49
Keel bij gehospitaliseerde patiënten = intubatiebuis .................................................................................... 49
Commensalen neus ....................................................................................................................................... 50
Pathogenen neus .......................................................................................................................................... 50
Middenoorontsteking ................................................................................................................................... 50
4
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller juliemouton. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $6.11. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
53340 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now