Summary
Summary Overview of all biomedical techniques
- Course
- Institution
Overview of all the techniques that are discussed during cases.
[Show more]
Seller
Follow
edmeesnijders
Reviews received
Content preview
All techniques MBS1002 When would you use this technique?
How to prepare sample?
How does this technique work?
MSI
Mass spectrometry imaging (MSI) = a technique used to visualize the
spatial distribution of biomolecules such as peptides, proteins, lipids or
other organic compounds by their molecular masses.
When would you use this technique?
For lipid research. Lipids are very valuable in cancer research and
eventually for treatment strategies. Alterations in the lipid composition of
tissue are seen in certain diseases, including cancer.
How to prepare sample?
Via cryosectioning. (see lecture)
How does this technique work?
See lecture.
PET scan
PET scans = produce detailed 3D images of the inside of the body.
When would you use this technique?
PET scan is an effective way to help identify a variety of conditions,
including cancer, heart disease and brain disorders.
How to prepare sample?
Avoid exercising 24 hours before the scan. Follow a special diet for 12 to
24 hours before the scan. Don't drink or eat anything, except water, for 6
hours before the scan.
How does this technique work?
A small amount of radioactive glucose (a sugar) is injected into a vein. The
PET scanner rotates around the body and makes a picture of where
glucose is being used in the brain. Malignant tumor cells show up brighter
in the picture because they are more active and take up more glucose
than normal cells do.
Histology
When would you use this technique?
Histology allows for the visualization of tissue structure and characteristic
changes the tissue may have undergone. Because of this, it is utilized in
medical diagnosis (cancer), scientific study, autopsy, and forensic
investigation.
Pagina 1 van 8
, How to prepare sample?
1. Fixation. Fixation is carried out immediately after the removal of the
sample to be observed.
2. Embedding. Embedding is the step that follows fixation in a fixative
solution. t consists in hardening the sample in a paraffin embedding
medium, in order to be able to carry out the sectioning.
3. Sectioning. Sectioning is performed using microtomy or cryotomy.
4. Staining and immunolabeling. Staining increases contrast to recognize
different things in the image.
5. Mounting. For adhesion.
Preparation of the slides using the paraffin technique can be time-
consuming; frozen slides are faster to prepare, but this can affect the
resolution, especially when using light microscopy.
How does this technique work?
see above.
Immunohistochemistry
When would you use this technique?
To determine the tissue distribution of an antigen of interest in health and
disease. Widely used for diagnosis of cancer. Because specific tumor
antigens are expressed de novo or up-regulated in certain cancers.
How to prepare sample?
Sample preparation may include processes such as fixation, dehydration,
embedding and sectioning.
Formalin-fixed tissue samples are usually embedded in paraffin to
maintain their natural shape and tissue architecture during long-term
storage and to facilitate sectioning prior to IHC. Such samples and the
sections prepared from them are usually referred to as formalin-fixed,
paraffin-embedded (FFPE) materials
Samples can be viewed by light/fluorescence microscopy.
How does this technique work?
ELISA
Pagina 2 van 8
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller edmeesnijders. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $8.65. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
56326 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now