Practicum verslag voor bouwstenen van het leven. Het verslag gaat over het fluorescerende eiwit GFP. Verslag is compleet en beoordeeld met een 7,6.
Opdracht op de Vu - studie gezondheid en leven
Isolatie en bepaling van
een fluorescerend eiwit
Onderzoek in het isoleren en aantonen van GFP, YFP, CFP en
mCherry en de verschillen tussen deze eiwitten.
Practicum verslag voor Bouwstenen van het leven
Paul Konig - Tu10 - 2556418
Britt Scheerman- Tu10 - 2573114
Inhoudsopgaven:
-Samenvatting 3
-Inleiding 3
Wat is GFP 3
Hoe ontstaat fluorescentie in GFP 3
Verschillende kleuren fluorescerende eiwitten 4
Fluorescerende eiwitten gebruikt in onderzoek 5
Ons onderzoek 5
, -Methode en materialen 7
Cultures aanenten (1) 7
Isolatie en zuivering van plasmide DNA 7
DNA analyse 8
Cultures aanenten voor eiwitexpressie (2) 8
Eiwitisolatie, eiwitbepaling 8
SDS Gelelectroforese: scheiden eiwitten 9
Spectrofotometer en fluorimeter 11
-Resultaten 12
Expressie van de plasmide 12
DNA analyse door middel van gelectroforese 12
De bepaling van het eiwitgehalte 13
Controleren van de zuiverheid 15
Het Absorptie- en fluorescentiespectrum 17
-Discussie 19
-Conclusie 20
-Bronvermelding 20
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