Summary
Summary Organelles inside eukaryotic cells
- Course
- Institution
The different organelles found in eukaryotic cells and their different functions
[Show more]
Seller
Follow
thrujanthayanesan
Content preview
Topic:Ultrastructure of eukaryotic cellsHomogenisation and Centrifugation
1st Step - Preparation
Place the tissue in cold, isotonic, buffered solution
- It is cold to reduce enzyme activity
- It being isotonic prevents organelles busting or shrinking as a
result of osmotic gain or loss (must have the same water
potential as the tissue)
- It is buffered to maintain a constant pH
2nd Step - Homogenisation
- The cells are broken up by a homogeniser to release
organelles
- The solution produced is called a homogenate
- The homogenate is then filtered
3rd Step - Centrifugation
- The homogenate is placed in a tube and spun at a high speed
creating a centrifugal force
- 1st spin - The heaviest organelles go to the bottom and form
as a pellet (Nucleus)
- 2nd spin - The fluid left from the first spin also known as the
(supernatant) is removed and spun again, the second heaviest
organelle will form as a pellet
Components of Eukaryotic cells
Nucleus contains Nucleus
DNA - Contains DNA, directs protein synthesis
- Surrounded by a double membrane called the nuclear
envelope protecting DNA containing gaps called nuclear pores
- Largest and most dense organelle
- Usually one per cell
Nucleolus
- Area of the nucleus which produces ribosomes
Mitochondria is Mitochondria
where cellular - Where cellular respiration occurs and releases energy for the
respiration cell
happens - Bound by a double membrane, the inner membrane fold to
form cristae
- Matrix is in the centre
- Has its own strand of DNA
Golgi apparatus Golgi apparatus
is where proteins - Looks like a stack of plates
are stores, - Stores, modifies and packages proteins
modified and Golgi vesicles
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller thrujanthayanesan. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.08. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
53068 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now