BIO 250L Lab 7 Microbial Genetics & Genetic Engineering (straighterline 2023)

Lab 7 Microbial Genetics & Genetic EngineeringBIO250L"Student Name:Daniel ElmoreAccess Code (located on the underside of the lid of your lab kit):AC-60PRCES"Pre-Lab Questions" 1.Which DNA nitrogenous bases pair with each other? Which bases are purines, and which arepyrimidines?Adenine and Thymine pair (A-T) and Guanine pairs with Cytosine (G-C). Adenine andGuanine are double-ringed and constitute the purines. Thymine and Cytosine arethesingle-ringed pyrimidines. 2.How is DNA information used to make proteins? What are the steps of this process?DNA is transcribed to mRNA with the use of the enzyme mRNA polymerases. mRNA canthen leave the nucleus (eukaryotes) into the cytoplasm (already in the cytoplasm inprokaryotes) where it encounters a ribosome. The mRNA is then translated usingtransfer (tRNA) to form new proteins. 3.Give an example of a scenario in which you would perform PCR vs a scenario in which you woulduse recombinant DNA technology.In recent history the SARS-CoV-2, the virus responsible for COVID-19, has shed a lot oflight on the use of both these technologies. PCR can be used in the detection of thevirus indicating infection. Recombinant DNA, for the same virus, is now being deployedin the synthesis of the vaccine for the virus and developing an immune response inhumans